Anti-RPL8 Antibody (CAB10042)
- SKU:
- CAB10042
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-RPL8 Antibody |
| Antibody SKU: | CAB10042 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human RPL8 (NP_000964.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:4000 IHC 1:50 - 1:200 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, 293T, A-375, HepG2, MCF7, Mouse lung, Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human RPL8 (NP_000964.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGRV IRGQ RKGA GSVF RAHV KHRK GAAR LRAV DFAE RHGY IKGI VKDI IHDP GRGA PLAK VVFR DPYR FKKR TELF IAAE GIHT GQFV YCGK KAQL NIGN VLPV GTMP EGTI VCCL EEKP GDRG KLAR ASGN YATV ISHN PETK KTRV KLPS GSKK VISS ANRA VVGV VAGG GRID KPIL KAGR AYHK YKAK RNCW PRVR GVAM NPVE HPFG GGNH QHIG KPST IRRD APAG RKVG LIAA RRTG RLRG TKTV QEKE N |
| Gene ID: | 6132 |
| Uniprot: | P62917 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 28kDa |
| Observed MW: | 28kDa |
| Synonyms: | RPL8, L8 |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| UniProt Protein Function: | RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. |
| UniProt Protein Details: | Protein type:RNA-binding; Translation; Ribosomal Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus Molecular Function:RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62917 |
| NCBI GenInfo Identifier: | 51702823 |
| NCBI Gene ID: | 6132 |
| NCBI Accession: | P62917.2 |
| UniProt Secondary Accession: | P62917,P25120, Q567Q7, Q969V7, Q9BWQ9, A8K094, D3DWN2 |
| UniProt Related Accession: | P62917 |
| Molecular Weight: | 28,025 Da |
| NCBI Full Name: | 60S ribosomal protein L8 |
| NCBI Synonym Full Names: | ribosomal protein L8 |
| NCBI Official Symbol: | RPL8 |
| NCBI Official Synonym Symbols: | L8 |
| NCBI Protein Information: | 60S ribosomal protein L8 |
| UniProt Protein Name: | 60S ribosomal protein L8 |
| Protein Family: | 60S ribosomal protein |
| UniProt Gene Name: | RPL8 |
| UniProt Entry Name: | RL8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL8 antibody (CAB10042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RPL8 Rabbit pAb (CAB10042) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using RPL8 Rabbit pAb (CAB10042) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using RPL8 Rabbit pAb (CAB10042) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using RPL8 antibody (CAB10042) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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