Anti-RBM3 Antibody (CAB5931)
- SKU:
- CAB5931
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-RBM3 Antibody |
| Antibody SKU: | CAB5931 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human RBM3 (NP_006734.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human RBM3 (NP_006734.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSE EGKL FVGG LNFN TDEQ ALED HFSS FGPI SEVV VVKD RETQ RSRG FGFI TFTN PEHA SVAM RAMN GESL DGRQ IRVD HAGK SARG TRGG GFGA HGRG RSYS RGGG DQGY GSGR YYDS RPGG YGYG YGRS RDYN GRNQ GGYD RYSG GNYR DNYD N |
| Gene ID: | 5935 |
| Uniprot: | P98179 |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, dendrite |
| Calculated MW: | 17kDa |
| Observed MW: | 17kDa |
| Synonyms: | RBM3, IS1-RNPL, RNPL |
| Background: | This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. |
| UniProt Protein Function: | RBM3: Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation. |
| UniProt Protein Details: | Protein type:Translation; RNA-binding; Ribosomal; RNA processing Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: cytoplasm; dendrite; nucleus; large ribosomal subunit Molecular Function:protein binding; RNA binding; nucleotide binding; ribosomal large subunit binding Biological Process: RNA processing; regulation of translation; miRNA-mediated gene silencing, production of miRNAs; positive regulation of translation; translation; response to cold |
| NCBI Summary: | This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P98179 |
| NCBI GenInfo Identifier: | 1710620 |
| NCBI Gene ID: | 5935 |
| NCBI Accession: | P98179.1 |
| UniProt Related Accession: | P98179 |
| Molecular Weight: | 157 |
| NCBI Full Name: | Putative RNA-binding protein 3 |
| NCBI Synonym Full Names: | RNA binding motif (RNP1, RRM) protein 3 |
| NCBI Official Symbol: | RBM3 |
| NCBI Official Synonym Symbols: | RNPL; IS1-RNPL |
| NCBI Protein Information: | putative RNA-binding protein 3; RNA-binding motif protein 3 |
| UniProt Protein Name: | Putative RNA-binding protein 3 |
| UniProt Synonym Protein Names: | RNA-binding motif protein 3; RNPL |
| Protein Family: | RNA-binding protein |
| UniProt Gene Name: | RBM3 |
| UniProt Entry Name: | RBM3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RBM3 antibody (CAB5931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RBM3 antibody (CAB5931) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM3 antibody (CAB5931) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using RBM3 antibody (CAB5931) at dilution of 1:100 (40x lens). |
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