Anti-RABIF Antibody (CAB15090)
- SKU:
- CAB15090
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-RABIF Antibody |
| Antibody SKU: | CAB15090 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human RABIF (NP_002862.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | DU145, mouse testis, rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human RABIF (NP_002862.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEPA EQPS ELVS AEGR NRKA VLCQ RCGS RVLQ PGTA LFSR RQLF LPSM RKKP ALSD GSNP DGDL LQEH WLVE DMFI FENV GFTK DVGN IKFL VCAD CEIG PIGW HCLD DKNS FYVA LERV SHE |
| Gene ID: | 5877 |
| Uniprot: | P47224 |
| Cellular Location: | |
| Calculated MW: | 13kDa |
| Observed MW: | 14kDa |
| Synonyms: | RABIF, MSS4, RASGFR3, RASGRF3 |
| Background: | This gene encodes a member of the SCE4/YPT1/RAB family of small GTP-binding proteins that are involved in the regulation of intracellular vesicular transport. This protein stimulates GTP-GDP exchange in SEC4, and to a lesser extent in YPT1 and RAB3A, and may play a general role in vesicular transport. |
| UniProt Protein Function: | RasGRF3: Guanine-nucleotide-releasing protein that acts on members of the SCE4/YPT1/RAB subfamily. Stimulates GDP release from both YPT1 and RAB3A, but is less active on these proteins than on the SEC4 protein. Might play a general role in vesicular transport. Belongs to the DSS4/MSS4 family. |
| UniProt Protein Details: | Protein type:GAPs; GAPs, Rab Chromosomal Location of Human Ortholog: 1q32.1 Molecular Function:protein binding; zinc ion binding; guanyl-nucleotide exchange factor activity Biological Process: protein transport; small GTPase mediated signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | This gene encodes a member of the SCE4/YPT1/RAB family of small GTP-binding proteins that are involved in the regulation of intracellular vesicular transport. This protein stimulates GTP-GDP exchange in SEC4, and to a lesser extent in YPT1 and RAB3A, and may play a general role in vesicular transport. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P47224 |
| NCBI GenInfo Identifier: | 19923149 |
| NCBI Gene ID: | 5877 |
| NCBI Accession: | NP_002862.2 |
| UniProt Related Accession: | P47224 |
| Molecular Weight: | |
| NCBI Full Name: | guanine nucleotide exchange factor MSS4 |
| NCBI Synonym Full Names: | RAB interacting factor |
| NCBI Official Symbol: | RABIF |
| NCBI Official Synonym Symbols: | MSS4; RASGFR3; RASGRF3 |
| NCBI Protein Information: | guanine nucleotide exchange factor MSS4 |
| UniProt Protein Name: | Guanine nucleotide exchange factor MSS4 |
| UniProt Synonym Protein Names: | Rab-interacting factor |
| Protein Family: | Probable phosphatidylinositol 4-phosphate 5-kinase |
| UniProt Gene Name: | RABIF |
| UniProt Entry Name: | MSS4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RABIF antibody (CAB15090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using RABIF Polyclonal Antibody (CAB15090) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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