Anti-PTBP1 Antibody (RACO0427)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- RACO0427
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | PTBP1 Antibody |
Product SKU: | RACO0427 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human PTBP1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806). |
Synonyms: | Polypyrimidine tract-binding protein 1 (PTB) (57 kDa RNA-binding protein PPTB-1) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I), PTBP1, PTB |
IHC image of RACO0427 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0427 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate PC-3 whole cell lysate K562 whole cell lysate HL-60 whole cell lysate MCF-7 whole cell lysate Jurkat whole cell lysate All lanes PTBP1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 58, 60, 60 kDa. Observed band size: 58 kDa. |
UniProt Protein Function: | hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; membrane; nucleolus Molecular Function:protein binding; poly-pyrimidine tract binding; RNA binding; nucleotide binding Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; negative regulation of muscle cell differentiation; gene expression; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome |
NCBI Summary: | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
UniProt Code: | P26599 |
NCBI GenInfo Identifier: | 131528 |
NCBI Gene ID: | 5725 |
NCBI Accession: | P26599.1 |
UniProt Secondary Accession: | P26599,Q9BUQ0, |
UniProt Related Accession: | P26599 |
Molecular Weight: | 531 |
NCBI Full Name: | Polypyrimidine tract-binding protein 1 |
NCBI Synonym Full Names: | polypyrimidine tract binding protein 1 |
NCBI Official Symbol: | PTBP1Â Â |
NCBI Official Synonym Symbols: | PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-IÂ Â |
NCBI Protein Information: | polypyrimidine tract-binding protein 1; hnRNP I; RNA-binding protein; 57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous |
UniProt Protein Name: | Polypyrimidine tract-binding protein 1 |
UniProt Synonym Protein Names: | 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I |
Protein Family: | Polypyrimidine tract-binding protein |
UniProt Gene Name: | PTBP1Â Â |
UniProt Entry Name: | PTBP1_HUMAN |