Anti-PRSS8 Antibody (CAB16419)
- SKU:
- CAB16419
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PRSS8 Antibody |
| Antibody SKU: | CAB16419 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human PRSS8 (NP_002764.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human PRSS8 (NP_002764.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TCNC LYNI DAKP EEPH FVQE DMVC AGYV EGGK DACQ GDSG GPLS CPVE GLWY LTGI VSWG DACG ARNR PGVY TLAS SYAS WIQS KVTE LQPR VVPQ TQES QPDS NLCG SHLA FSSA PAQG LLRP ILFL PLGL ALGL LSPW LSEH |
| Gene ID: | 5652 |
| Uniprot: | Q16651 |
| Cellular Location: | Cell membrane, Secreted, Single-pass membrane protein, extracellular space, extracellular space |
| Calculated MW: | 30kDa/36kDa |
| Observed MW: | 36kDa |
| Synonyms: | PRSS8, CAP1, PROSTASIN, prostasin |
| Background: | This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation. |
| UniProt Protein Function: | PRSS8: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). Belongs to the peptidase S1 family. |
| UniProt Protein Details: | Protein type:EC 3.4.21.-; Membrane protein, integral; Protease Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: extracellular space; plasma membrane; extracellular region; integral to membrane Molecular Function:protein binding; serine-type peptidase activity; serine-type endopeptidase activity Biological Process: proteolysis |
| NCBI Summary: | This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16651 |
| NCBI GenInfo Identifier: | 2833277 |
| NCBI Gene ID: | 5652 |
| NCBI Accession: | Q16651.1 |
| UniProt Secondary Accession: | Q16651,Q9UCA3, B4DWP2, |
| UniProt Related Accession: | Q16651 |
| Molecular Weight: | 30,519 Da |
| NCBI Full Name: | Prostasin |
| NCBI Synonym Full Names: | protease, serine, 8 |
| NCBI Official Symbol: | PRSS8 |
| NCBI Official Synonym Symbols: | CAP1; PROSTASIN |
| NCBI Protein Information: | prostasin; serine protease 8; channel-activating protease 1 |
| UniProt Protein Name: | Prostasin |
| UniProt Synonym Protein Names: | Channel-activating protease 1; CAP1; Serine protease 8Cleaved into the following 2 chains:Prostasin light chain; Prostasin heavy chain |
| Protein Family: | Prostasin |
| UniProt Gene Name: | PRSS8 |
| UniProt Entry Name: | PRSS8_HUMAN |
 | Western blot analysis of extracts of Mouse kidney, using PRSS8 Rabbit pAb (CAB16419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U-2 OS cells using PRSS8 antibody (CAB16419) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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