Anti-PITRM1 Antibody (CAB8362)
- SKU:
- CAB8362
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PITRM1 Antibody |
| Antibody SKU: | CAB8362 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 758-1037 of human PITRM1 (NP_055704.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, A-549, MCF7, U-87MG, Mouse liver, Mouse heart, Mouse kidney, Rat liver, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 758-1037 of human PITRM1 (NP_055704.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IKPI LRKL PRIK KHLL NGDN MRCS VNAT PQQM PQTE KAVE DFLR SIGR SKKE RRPV RPHT VEKP VPSS SGGD AHVP HGSQ VIRK LVME PTFK PWQM KTHF LMPF PVNY VGEC IRTV PYTD PDHA SLKI LARL MTAK FLHT EIRE KGGA YGGG AKLS HNGI FTLY SYRD PNTI ETLQ SFGK AVDW AKSG KFTQ QDID EAKL SVFS TVDA PVAP SDKG MDHF LYGL SDEM KQAH REQL FAVS HDKL LAVS DRYL GTGK STHG LAIL GPEN PKIA KDPS WIIQ |
| Gene ID: | 10531 |
| Uniprot: | Q5JRX3 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 106kDa/117kDa |
| Observed MW: | 117kDa |
| Synonyms: | PITRM1, MP1, PreP |
| Background: | The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease. |
| UniProt Protein Function: | PITRM1: ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Belongs to the peptidase M16 family. PreP subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Protease; EC 3.4.24.-; Activator Chromosomal Location of Human Ortholog: 10p15.2 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:enzyme activator activity; metalloendopeptidase activity; zinc ion binding Biological Process: protein processing; proteolysis |
| UniProt Code: | Q5JRX3 |
| NCBI GenInfo Identifier: | 485956568 |
| NCBI Gene ID: | 10531 |
| NCBI Accession: | Q5JRX3.3 |
| UniProt Secondary Accession: | Q5JRX3,O95204, Q2M2G6, Q4VBR1, Q5JRW7, Q7L5Z7, Q9BSI6 Q9BVJ5, B3KMJ6, B4E0J8, C9JSL2, E7ES23, |
| UniProt Related Accession: | Q5JRX3 |
| Molecular Weight: | 106,065 Da |
| NCBI Full Name: | Presequence protease, mitochondrial |
| NCBI Synonym Full Names: | pitrilysin metallopeptidase 1 |
| NCBI Official Symbol: | PITRM1 |
| NCBI Official Synonym Symbols: | MP1; PreP |
| NCBI Protein Information: | presequence protease, mitochondrial |
| UniProt Protein Name: | Presequence protease, mitochondrial |
| UniProt Synonym Protein Names: | Pitrilysin metalloproteinase 1; Metalloprotease 1; hMP1 |
| UniProt Gene Name: | PITRM1 |
| UniProt Entry Name: | PREP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PITRM1 antibody (CAB8362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat heart using PITRM1 antibody (CAB8362) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using Anti-PITRM1 Polyclonal Antibody (CAB8362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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