Anti-PIM3 Antibody (CAB17285)
- SKU:
- CAB17285
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-PIM3 Antibody |
| Antibody SKU: | CAB17285 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 247-326 of human PIM3 (NP_001001852.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | SKOV3, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 247-326 of human PIM3 (NP_001001852.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QDEE ILRG RLLF RRRV SPEC QQLI RWCL SLRP SERP SLDQ IAAH PWML GADG GVPE SCDL RLCT LDPD DVAS TTSS SESL |
| Gene ID: | 415116 |
| Uniprot: | Q86V86 |
| Cellular Location: | |
| Calculated MW: | 37kDa |
| Observed MW: | 37kDa |
| Synonyms: | PIM3, pim-3 |
| Background: | The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. |
| UniProt Protein Function: | Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth. |
| UniProt Code: | Q86V86 |
| NCBI GenInfo Identifier: | 224591416 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_001001852 |
| Molecular Weight: | 35.863 |
| NCBI Full Name: | serine/threonine-protein kinase pim-3 |
| UniProt Protein Name: | Serine/threonine-protein kinase pim-3 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | PIM3 |
 | Western blot analysis of extracts of various cells, using PIM3 antibody (CAB17285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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