Anti-PI4KB Antibody (CAB16416)
- SKU:
- CAB16416
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-PI4KB Antibody |
| Antibody SKU: | CAB16416 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-801 of human PI4KB (NP_001185702). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse liver, Rat brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-801 of human PI4KB (NP_001185702). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AFQV LKQL QSIW EQER VPLW IKPY KILV ISAD SGMI EPVV NAVS IHQV KKQS QLSL LDYF LQEH GSYT TEAF LSAQ RNFV QSCA GYCL VCYL LQVK DRHN GNIL LDAE GHII HIDF GFIL SSSP RNLG FETS AFKL TTEF VDVM GGLD GDMF NYYK MLML QGLI AARK HMDK VVQI VEIM QQGS QLPC FHGS STIR NLKE RFHM SMTE EQLQ LLVE QMVD GSMR SITT KLYD GFQY LTNG IM |
| Gene ID: | 5298 |
| Uniprot: | Q9UBF8 |
| Cellular Location: | Cytoplasm, Endomembrane system, Golgi apparatus, Mitochondrion outer membrane, Peripheral membrane protein, Rough endoplasmic reticulum membrane, perinuclear region |
| Calculated MW: | 54kDa/89kDa/91kDa |
| Observed MW: | 91kDa |
| Synonyms: | PI4KB, NPIK, PI4K-BETA, PI4K92, PI4KBETA, PI4KIIIBETA, PIK4CB |
| Background: |
| UniProt Protein Function: | PIK4CB: a member of the PI3/PI4 kinase family. Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex. May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Three splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; EC 2.7.1.67 Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoplasm; cytosol; endosome; Golgi membrane Molecular Function:1-phosphatidylinositol 4-kinase activity; protein binding Biological Process: phosphatidylinositol biosynthetic process; receptor-mediated endocytosis; signal transduction |
| UniProt Code: | Q9UBF8 |
| NCBI GenInfo Identifier: | 38372507 |
| NCBI Gene ID: | 5298 |
| NCBI Accession: | Q9UBF8.1 |
| UniProt Secondary Accession: | Q9UBF8,O15096, P78405, Q5VWB9, Q5VWC0, Q5VWC1, Q9BWR6 B4DGI2, |
| UniProt Related Accession: | Q9UBF8 |
| Molecular Weight: | 54,976 Da |
| NCBI Full Name: | Phosphatidylinositol 4-kinase beta |
| NCBI Synonym Full Names: | phosphatidylinositol 4-kinase beta |
| NCBI Official Symbol: | PI4KB |
| NCBI Official Synonym Symbols: | NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA |
| NCBI Protein Information: | phosphatidylinositol 4-kinase beta |
| UniProt Protein Name: | Phosphatidylinositol 4-kinase beta |
| UniProt Synonym Protein Names: | NPIK; PI4K92 |
| Protein Family: | Phosphatidylinositol 4-kinase |
| UniProt Gene Name: | PI4KB |
| UniProt Entry Name: | PI4KB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PI4KB antibody (CAB16416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using PI4KB antibody (CAB16416) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using PI4KB antibody (CAB16416) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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