Anti-PHC2 Antibody (CAB14610)
- SKU:
- CAB14610
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PHC2 Antibody |
| Antibody SKU: | CAB14610 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-323 of human PHC2 (NP_004418.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | DU145, HT-29, HeLa, Jurkat, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-323 of human PHC2 (NP_004418.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HFLP SEPT KWNV EDVY EFIR SLPG CQEI AEEF RAQE IDGQ ALLL LKED HLMS AMNI KLGP ALKI YARI SMLK DS |
| Gene ID: | 1912 |
| Uniprot: | Q8IXK0 |
| Cellular Location: | Nucleus |
| Calculated MW: | 30kDa/35kDa/87kDa/90kDa |
| Observed MW: | 45kDa |
| Synonyms: | PHC2, EDR2, HPH2, PH2 |
| Background: | In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PHC2: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: heterochromatin; nucleoplasm; nucleus; PcG protein complex Molecular Function:DNA binding; identical protein binding; protein binding; zinc ion binding Biological Process: multicellular organismal development; protein sumoylation; spermatogenesis |
| NCBI Summary: | In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8IXK0 |
| NCBI GenInfo Identifier: | 219520710 |
| NCBI Gene ID: | 1912 |
| NCBI Accession: | AAI44122.1 |
| UniProt Secondary Accession: | Q8IXK0,Q2TAL3, Q5T0C1, Q6NUJ6, Q6ZQR1, Q8N306, Q8TAG8 Q96BL4, Q9Y4Y7, A1L4Q1, A8KA40, D3DPR2, |
| UniProt Related Accession: | Q8IXK0 |
| Molecular Weight: | 40kd |
| NCBI Full Name: | PHC2 protein |
| NCBI Synonym Full Names: | polyhomeotic homolog 2 |
| NCBI Official Symbol: | PHC2 |
| NCBI Official Synonym Symbols: | PH2; EDR2; HPH2 |
| NCBI Protein Information: | polyhomeotic-like protein 2 |
| UniProt Protein Name: | Polyhomeotic-like protein 2 |
| UniProt Synonym Protein Names: | Early development regulatory protein 2 |
| Protein Family: | Polyhomeotic-like protein |
| UniProt Gene Name: | PHC2 |
| UniProt Entry Name: | PHC2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHC2 antibody (CAB14610) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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