Anti-PHC1 Antibody (CAB5843)
- SKU:
- CAB5843
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PHC1 Antibody |
| Antibody SKU: | CAB5843 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 750-940 of human PHC1 (NP_004417.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 750-940 of human PHC1 (NP_004417.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FPVG CSQL LKES EKPL QTGL PTGL TENQ SGGP LGVD SPSA ELDK KANL LKCE YCGK YAPA EQFR GSKR FCSM TCAK RYNV SCSH QFRL KRKK MKEF QEAN YARV RRRG PRRS SSDI ARAK IQGK CHRG QEDS SRGS DNSS YDEA LSPT SPGP LSVR AGHG ERDL GNPN TAPP TPEL HGIN PVFL SSNP SRW |
| Gene ID: | 1911 |
| Uniprot: | P78364 |
| Cellular Location: | Nucleus |
| Calculated MW: | 105kDa |
| Observed MW: | 125kDa/135kDa |
| Synonyms: | PHC1, EDR1, HPH1, MCPH11, RAE28 |
| Background: | This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. |
| UniProt Protein Function: | PHC1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 12p13 Cellular Component: nucleoplasm; nuclear body; sex chromatin; PcG protein complex; nucleus Molecular Function:DNA binding; zinc ion binding; chromatin binding Biological Process: multicellular organismal development; histone ubiquitination Disease: Microcephaly 11, Primary, Autosomal Recessive |
| NCBI Summary: | This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P78364 |
| NCBI GenInfo Identifier: | 224471881 |
| NCBI Gene ID: | 1911 |
| NCBI Accession: | P78364.3 |
| UniProt Secondary Accession: | P78364,Q8WVM3, Q9BU63, D3DUV4, |
| UniProt Related Accession: | P78364 |
| Molecular Weight: | Calculated MW: 105kDaObserved MW: 125kDa/135kDa |
| NCBI Full Name: | Polyhomeotic-like protein 1 |
| NCBI Synonym Full Names: | polyhomeotic homolog 1 (Drosophila) |
| NCBI Official Symbol: | PHC1 |
| NCBI Official Synonym Symbols: | EDR1; HPH1; RAE28; MCPH11 |
| NCBI Protein Information: | polyhomeotic-like protein 1; polyhomeotic-like 1; early development regulatory protein 1; early development regulator 1 (homolog of polyhomeotic 1) |
| UniProt Protein Name: | Polyhomeotic-like protein 1 |
| UniProt Synonym Protein Names: | Early development regulatory protein 1 |
| Protein Family: | Polyhomeotic-like protein |
| UniProt Gene Name: | PHC1 |
| UniProt Entry Name: | PHC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHC1 Antibody (CAB5843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human adenomyosis using PHC1 antibody (CAB5843) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using PHC1 antibody (CAB5843) at dilution of 1:100 (40x lens). |
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