Anti-PDZK1 Antibody (CAB5321)
- SKU:
- CAB5321
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PDZK1 Antibody |
| Antibody SKU: | CAB5321 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDZK1 (NP_001188254.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDZK1 (NP_001188254.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTST FNPR ECKL SKQE GQNY GFFL RIEK DTEG HLVR VVEK CSPA EKAG LQDG DRVL RING VFVD KEEH MQVV DLVR KSGN SVTL LVLD GDSY EKAV KTRV DLKE LGQS QKEQ GLSD NILS PVMN GGVQ TWTQ PRLC YLVK EGGS YGFS LKTV QGKK GVYM TDIT PQGV AMRA GVLA DDHL IEVN GENV EDAS HEEV VEKV KKSG SRVM FLLV DKET DKRH VEQK IQFK RETA SLKL LPHQ PRIV EMKK GSNG YGFY LRAG |
| Gene ID: | 5174 |
| Uniprot: | Q5T2W1 |
| Cellular Location: | Cell membrane, Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 44kDa/57kDa |
| Observed MW: | 67kDa |
| Synonyms: | PDZK1, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1 |
| Background: | This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 1q21 Cellular Component: brush border membrane; plasma membrane Molecular Function:PDZ domain binding; protein binding; transporter activity Biological Process: carnitine transport; cell proliferation; transport |
| NCBI Summary: | This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | Q5T2W1 |
| NCBI GenInfo Identifier: | 73621372 |
| NCBI Gene ID: | 5174 |
| NCBI Accession: | Q5T2W1.2 |
| UniProt Secondary Accession: | Q5T2W1,O60450, Q5T5P6, Q9BQ41, B4DPB9, E7EU02, |
| UniProt Related Accession: | Q5T2W1 |
| Molecular Weight: | 44,665 Da |
| NCBI Full Name: | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 |
| NCBI Synonym Full Names: | PDZ domain containing 1 |
| NCBI Official Symbol: | PDZK1 |
| NCBI Official Synonym Symbols: | CAP70; CLAMP; PDZD1; NHERF3; NHERF-3 |
| NCBI Protein Information: | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 |
| UniProt Protein Name: | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 |
| UniProt Synonym Protein Names: | CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3 |
| Protein Family: | PDZK1-interacting protein |
| UniProt Gene Name: | PDZK1 |
| UniProt Entry Name: | NHRF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDZK1 antibody (CAB5321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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