Anti-P4HA3 Antibody (CAB13767)
- SKU:
- CAB13767
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-P4HA3 Antibody |
| Antibody SKU: | CAB13767 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human P4HA3 (NP_878907.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human P4HA3 (NP_878907.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DPER AAAR GDTF SALT SVAR ALAP ERRL LGLL RRYL RGEE ARLR DLTR FYDK VLSL HEDS TTPV ANPL LAFT LIKR LQSD WRNV VHSL EAS |
| Gene ID: | 283208 |
| Uniprot: | Q7Z4N8 |
| Cellular Location: | Endoplasmic reticulum lumen |
| Calculated MW: | 28kDa/61kDa/67kDa |
| Observed MW: | 61kDa |
| Synonyms: | P4HA3 |
| Background: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | P4HA3: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.11.2; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q13.4 Molecular Function:protein binding |
| NCBI Summary: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | Q7Z4N8 |
| NCBI GenInfo Identifier: | 74738714 |
| NCBI Gene ID: | 283208 |
| NCBI Accession: | Q7Z4N8.1 |
| UniProt Secondary Accession: | Q7Z4N8,Q5EBL3, Q5JPA9, A0AV13, B4DUD3, |
| UniProt Related Accession: | Q7Z4N8 |
| Molecular Weight: | 67,591 Da |
| NCBI Full Name: | Prolyl 4-hydroxylase subunit alpha-3 |
| NCBI Synonym Full Names: | prolyl 4-hydroxylase subunit alpha 3 |
| NCBI Official Symbol: | P4HA3 |
| NCBI Protein Information: | prolyl 4-hydroxylase subunit alpha-3 |
| UniProt Protein Name: | Prolyl 4-hydroxylase subunit alpha-3 |
| UniProt Synonym Protein Names: | Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3 |
| Protein Family: | Prolyl 4-hydroxylase |
| UniProt Gene Name: | P4HA3 |
| UniProt Entry Name: | P4HA3_HUMAN |
 | Western blot analysis of extracts of 293T cells, using P4HA3 antibody (CAB13767) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using P4HA3 Rabbit pAb (CAB13767) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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