Anti-OPA1 Antibody (CAB9833)
- SKU:
- CAB9833
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-OPA1 Antibody |
| Antibody SKU: | CAB9833 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 661-960 of human OPA1 (NP_056375.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, U-251MG, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 661-960 of human OPA1 (NP_056375.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TDKQ LPNK AVEV AWET LQEE FSRF MTEP KGKE HDDI FDKL KEAV KEES IKRH KWND FAED SLRV IQHN ALED RSIS DKQQ WDAA IYFM EEAL QARL KDTE NAIE NMVG PDWK KRWL YWKN RTQE QCVH NETK NELE KMLK CNEE HPAY LASD EITT VRKN LESR GVEV DPSL IKDT WHQV YRRH FLKT ALNH CNLC RRGF YYYQ RHFV DSEL ECND VVLF WRIQ RMLA ITAN TLRQ QLTN TEVR RLEK NVKE VLED FAED GEKK IKLL TGKR VQLA EDLK KVRE IQEK LDAF IEAL HQEK |
| Gene ID: | 4976 |
| Uniprot: | O60313 |
| Cellular Location: | Mitochondrion inner membrane, Mitochondrion intermembrane space, Single-pass membrane protein |
| Calculated MW: | 111kDa/115kDa |
| Observed MW: | 80-100KDa |
| Synonyms: | OPA1, BERHS, MGM1, MTDPS14, NPG, NTG, largeG |
| Background: | This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | OPA1: a dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Defects in OPA1 are the cause of optic atrophy 1 and deafness. Eight splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Apoptosis; EC 3.6.5.5; Hydrolase; Membrane protein, integral; Mitochondrial Chromosomal Location of Human Ortholog: 3q29 Cellular Component: cytosol; dendrite; membrane; mitochondrial crista; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrion; nucleoplasm Molecular Function:GTPase activity; microtubule binding; protein binding Biological Process: axon transport of mitochondrion; inner mitochondrial membrane organization and biogenesis; mitochondrial fission; mitochondrial fusion; mitochondrial genome maintenance; mitochondrion organization and biogenesis; regulation of apoptosis; visual perception Disease: Behr Syndrome; Glaucoma, Normal Tension, Susceptibility To; Mitochondrial Dna Depletion Syndrome 14 (cardioencephalomyopathic Type); Optic Atrophy 1; Optic Atrophy With Or Without Deafness, Ophthalmoplegia, Myopathy, Ataxia, And Neuropathy |
| NCBI Summary: | This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| UniProt Code: | O60313 |
| NCBI GenInfo Identifier: | 215274226 |
| NCBI Gene ID: | 4976 |
| NCBI Accession: | O60313.3 |
| UniProt Secondary Accession: | O60313,D3DNW4, |
| UniProt Related Accession: | O60313 |
| Molecular Weight: | 112kDa |
| NCBI Full Name: | Dynamin-like 120 kDa protein, mitochondrial |
| NCBI Synonym Full Names: | OPA1, mitochondrial dynamin like GTPase |
| NCBI Official Symbol: | OPA1 |
| NCBI Official Synonym Symbols: | NPG; NTG; MGM1; BERHS; largeG; MTDPS14 |
| NCBI Protein Information: | dynamin-like 120 kDa protein, mitochondrial |
| UniProt Protein Name: | Dynamin-like 120 kDa protein, mitochondrial |
| UniProt Synonym Protein Names: | Optic atrophy protein 1 |
| Protein Family: | Dynamin-like 120 kDa protein |
| UniProt Gene Name: | OPA1 |
 | Western blot analysis of extracts of various cell lines, using OPA1 Rabbit pAb (CAB9833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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