Anti-NRXN1 Antibody (CAB10066)
- SKU:
- CAB10066
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NRXN1 Antibody |
| Antibody SKU: | CAB10066 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 31-310 of human NRXN1 (NP_001129131.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HepG2, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 31-310 of human NRXN1 (NP_001129131.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LEFP GAEG QWTR FPKW NACC ESEM SFQL KTRS ARGL VLYF DDEG FCDF LELI LTRG GRLQ LSFS IFCA EPAT LLAD TPVN DGAW HSVR IRRQ FRNT TLFI DQVE AKWV EVKS KRRD MTVF SGLF VGGL PPEL RAAA LKLT LASV RERE PFKG WIRD VRVN SSQV LPVD SGEV KLDD EPPN SGGG SPCE AGEE GEGG VCLN GGVC SVVD DQAV CDCS RTGF RGKD CSQE IKFG LQCV LPVL LHDN DQGK YCCI NTAK PLTE KDNN VEGL AHLM MGDQ GKSK |
| Gene ID: | 9378 |
| Uniprot: | P58400/Q9ULB1 |
| Cellular Location: | Cell junction, Cell membrane, Single-pass type I membrane protein, synapse |
| Calculated MW: | 15kDa/46kDa/161kDa/164kDa/169kDa |
| Observed MW: | 162kDa |
| Synonyms: | NRXN1, Hs.22998, PTHSL2, SCZD17 |
| Background: | This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission, and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3, 000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. The RefSeq project has decided to create only a few representative transcript variants of the multitude that are possible. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2. |
| UniProt Protein Function: | NRXN1 iso5: Cell surface protein involved in cell-cell-interactions, excytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)- triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latroxin from spider venom. Belongs to the neurexin family. 4 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 2p16.3 Cellular Component: cell soma; cell surface; endoplasmic reticulum; integral to plasma membrane; nuclear membrane; plasma membrane; presynaptic membrane; vesicle Molecular Function:acetylcholine receptor binding; calcium channel regulator activity; calcium ion binding; cell adhesion molecule binding; protein binding; receptor activity Biological Process: adult behavior; axon guidance; learning; neuromuscular process controlling balance; neuron adhesion; neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; social behavior; synaptic transmission; synaptogenesis; vocal learning Disease: Autism |
| NCBI Summary: | This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission, and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. The RefSeq project has decided to create only a few representative transcript variants of the multitude that are possible. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P58400 |
| NCBI GenInfo Identifier: | 322510054 |
| NCBI Gene ID: | 9378 |
| NCBI Accession: | P58400.2 |
| UniProt Related Accession: | Q9ULB1,P58400 |
| Molecular Weight: | 161,883 Da |
| NCBI Full Name: | Neurexin-1-beta |
| NCBI Synonym Full Names: | neurexin 1 |
| NCBI Official Symbol: | NRXN1 |
| NCBI Official Synonym Symbols: | PTHSL2; SCZD17; Hs.22998 |
| NCBI Protein Information: | neurexin-1 |
| UniProt Protein Name: | Neurexin-1-beta |
| UniProt Synonym Protein Names: | Neurexin I-beta |
| Protein Family: | Neurexin |
| UniProt Gene Name: | NRXN1 |
| UniProt Entry Name: | NRX1B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NRXN1 antibody (CAB10066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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