Anti-NLRC4 Antibody (CAB7382)
- SKU:
- CAB7382
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-NLRC4 Antibody |
| Antibody SKU: | CAB7382 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NLRC4 (NP_067032.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, MCF7, HL-60, Mouse kidney, Mouse heart, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NLRC4 (NP_067032.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNFI KDNS RALI QRMG MTVI KQIT DDLF VWNV LNRE EVNI ICCE KVEQ DAAR GIIH MILK KGSE SCNL FLKS LKEW NYPL FQDL NGQS LFHQ TSEG DLDD LAQD LKDL YHTP SFLN FYPL GEDI DIIF NLKS TFTE PVLW RKDQ HHHR VEQL TLNG LLQA LQSP CIIE GESG KGKS TLLQ RIAM LWGS GKCK ALTK FKFV FFLR LSRA QGGL FETL CDQL LDIP GTIR KQTF MAML LKLR QRVL FLLD GY |
| Gene ID: | 58484 |
| Uniprot: | Q9NPP4 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 10kDa/18kDa/40kDa/116kDa |
| Observed MW: | 116kDa |
| Synonyms: | NLRC4, AIFEC, CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, FCAS4, IPAF |
| Background: | This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis Chromosomal Location of Human Ortholog: 2p22.3 Cellular Component: cytosol; nucleus; spindle microtubule Molecular Function:ATP binding; cysteine protease inhibitor activity; identical protein binding; magnesium ion binding; protein binding; protein homodimerization activity; ubiquitin-protein ligase activity Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; caspase activation; defense response to bacterium; detection of bacterium; inflammatory response; innate immune response; interleukin-1 beta secretion; positive regulation of apoptosis; protein homooligomerization; regulation of apoptosis; regulation of signal transduction Disease: Autoinflammation With Infantile Enterocolitis; Familial Cold Autoinflammatory Syndrome 4 |
| NCBI Summary: | This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| UniProt Code: | Q9NPP4 |
| NCBI GenInfo Identifier: | 20138032 |
| NCBI Gene ID: | 58484 |
| NCBI Accession: | Q9NPP4.2 |
| UniProt Secondary Accession: | Q9NPP4,Q96J81, Q96J82, Q96J83, A8K9F8, B2RBQ3, B3KTF0 D6W580, |
| UniProt Related Accession: | Q9NPP4 |
| Molecular Weight: | 10,646 Da |
| NCBI Full Name: | NLR family CARD domain-containing protein 4 |
| NCBI Synonym Full Names: | NLR family CARD domain containing 4 |
| NCBI Official Symbol: | NLRC4 |
| NCBI Official Synonym Symbols: | CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1 |
| NCBI Protein Information: | NLR family CARD domain-containing protein 4 |
| UniProt Protein Name: | NLR family CARD domain-containing protein 4 |
| UniProt Synonym Protein Names: | CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf |
| Protein Family: | NLR family CARD domain-containing protein |
| UniProt Gene Name: | NLRC4 |
| UniProt Entry Name: | NLRC4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NLRC4 antibody (CAB7382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using NLRC4 antibody (CAB7382) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using NLRC4 Rabbit pAb (CAB7382) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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