Anti-Nectin 2/CD112 Antibody (CAB5378)
- SKU:
- CAB5378
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Nectin 2/CD112 Antibody |
| Antibody SKU: | CAB5378 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 382-479 of human Nectin 2/CD112 (NP_002847.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | MCF-7, SW620, A-549, SKOV3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 382-479 of human Nectin 2/CD112 (NP_002847.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FILL RVRR RRKS PGGA GGGA SGDG GFYD PKAQ VLGN GDPV FWTP VVPG PMEP DGKD EEEE EEEE KAEK GLML PPPP ALED DMES QLDG SLIS RRAV YV |
| Gene ID: | 5819 |
| Uniprot: | Q92692 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 51kDa/57kDa |
| Observed MW: | 72kDa |
| Synonyms: | NECTIN2, CD112, HVEB, PRR2, PVRL2, PVRR2, nectin-2 |
| Background: | This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| UniProt Protein Function: | Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705). Probable cell adhesion protein (PubMed:9657005). |
| NCBI Summary: | This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92692 |
| NCBI GenInfo Identifier: | 12643789 |
| NCBI Gene ID: | 5819 |
| NCBI Accession: | Q92692.1 |
| UniProt Secondary Accession: | Q92692,O75455, Q6IBI6, Q96J29, A8K5L5, |
| UniProt Related Accession: | Q92692 |
| Molecular Weight: | |
| NCBI Full Name: | Nectin-2 |
| NCBI Synonym Full Names: | nectin cell adhesion molecule 2 |
| NCBI Official Symbol: | NECTIN2 |
| NCBI Official Synonym Symbols: | HVEB; PRR2; CD112; PVRL2; PVRR2 |
| NCBI Protein Information: | nectin-2 |
| UniProt Protein Name: | Nectin-2 |
| UniProt Synonym Protein Names: | Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Poliovirus receptor-related protein 2; CD_antigen: CD112 |
| UniProt Gene Name: | PVRL2 |
| UniProt Entry Name: | PVRL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Nectin 2/CD112 antibody (CAB5378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunofluorescence analysis of HeLa cells using Nectin 2/CD112 antibody (CAB5378). Blue: DAPI for nuclear staining. |
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