Anti-NDUFS6 Antibody (CAB3985)
- SKU:
- CAB3985
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NDUFS6 Antibody |
| Antibody SKU: | CAB3985 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-124 of human NDUFS6 (NP_004544.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse liver, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-124 of human NDUFS6 (NP_004544.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GVRV SPTG EKVT HTGQ VYDD KDYR RIRF VGRQ KEVN ENFA IDLI AEQP VSEV ETRV IACD GGGG ALGH PKVY INLD KETK TGTC GYCG LQFR QHHH |
| Gene ID: | 4726 |
| Uniprot: | O75380 |
| Cellular Location: | Matrix side, Mitochondrion inner membrane, Peripheral membrane protein |
| Calculated MW: | 13kDa |
| Observed MW: | 14kDa |
| Synonyms: | NDUFS6, CI-13kA, CI-13kD-A, CI13KDA |
| Background: | This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders. |
| UniProt Protein Function: | NDUFS6: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFS6 subunit family. |
| UniProt Protein Details: | Protein type:EC 1.6.99.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.6.5.3 Chromosomal Location of Human Ortholog: 5p15.33 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.[provided by RefSeq, Oct 2009] |
| UniProt Code: | O75380 |
| NCBI GenInfo Identifier: | 6093600 |
| NCBI Gene ID: | 4726 |
| NCBI Accession: | O75380.1 |
| UniProt Related Accession: | O75380 |
| Molecular Weight: | 13,712 Da |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit S6 |
| NCBI Official Symbol: | NDUFS6 |
| NCBI Official Synonym Symbols: | CI-13kA; CI13KDA; CI-13kD-A |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-13kD-A; CI-13kD-A; NADH-ubiquinone oxidoreductase 13 kDa-A subunit |
| UniProt Gene Name: | NDUFS6 |
| UniProt Entry Name: | NDUS6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFS6 antibody (CAB3985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |