Anti-NCS1 Antibody (CAB6455)
- SKU:
- CAB6455
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NCS1 Antibody |
| Antibody SKU: | CAB6455 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCS1 (NP_055101.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SW620, MCF-7, U-251MG, Mouse bone marrow, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCS1 (NP_055101.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGKS NSKL KPEV VEEL TRKT YFTE KEVQ QWYK GFIK DCPS GQLD AAGF QKIY KQFF PFGD PTKF ATFV FNVF DENK DGRI EFSE FIQA LSVT SRGT LDEK LRWA FKLY DLDN DGYI TRNE MLDI VDAI YQMV GNTV ELPE EENT PEKR VDRI FAMM DKNA DGKL TLQE FQEG SKAD PSIV QALS LYDG LV |
| Gene ID: | 23413 |
| Uniprot: | P62166 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Golgi stack membrane, Lipid-anchor, Membrane, Peripheral membrane protein, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse |
| Calculated MW: | 19kDa/21kDa |
| Observed MW: | 20kDa |
| Synonyms: | NCS1, FLUP, FREQ |
| Background: | This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FREQ: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with IL1RAPL1. Belongs to the recoverin family. |
| UniProt Protein Details: | Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 9q34 Cellular Component: cytoplasm; intracellular membrane-bound organelle; plasma membrane Molecular Function:calcium ion binding; protein binding; voltage-gated calcium channel activity |
| NCBI Summary: | This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62166 |
| NCBI GenInfo Identifier: | 49065666 |
| NCBI Gene ID: | 23413 |
| NCBI Accession: | P62166.2 |
| UniProt Secondary Accession: | P62166,P36610, Q9UK26, E9PAY3, |
| UniProt Related Accession: | P62166 |
| Molecular Weight: | 19,728 Da |
| NCBI Full Name: | Neuronal calcium sensor 1 |
| NCBI Synonym Full Names: | neuronal calcium sensor 1 |
| NCBI Official Symbol: | NCS1 |
| NCBI Official Synonym Symbols: | FLUP; FREQ |
| NCBI Protein Information: | neuronal calcium sensor 1 |
| UniProt Protein Name: | Neuronal calcium sensor 1 |
| UniProt Synonym Protein Names: | Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein |
| Protein Family: | Frequenin |
| UniProt Gene Name: | NCS1 |
| UniProt Entry Name: | NCS1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCS1 antibody (CAB6455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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