Anti-MRPS9 항체 (CAB11729)

Antibody Name:	Anti-MRPS9 Antibody
Antibody SKU:	CAB11729
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-396 of human MRPS9 (NP_872578.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, U-87MG, A-431, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-396 of human MRPS9 (NP_872578.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EEEF VQRF RRSV TLES KKQL IEPV QYDE QGMA FSKS EGKR KTAK AEAI VYKH GSGR IKVN GIDY QLYF PITQ DREQ LMFP FHFV DRLG KHDV TCTV SGGG RSAQ AGAI RLAM AKAL CSFV TEDE VEWM RQAG LLTT DPRV RERK KPGQ EGAR RKFT WKKR
Gene ID:	64965
Uniprot:	P82933
Cellular Location:	Mitochondrion
Calculated MW:	45kDa
Observed MW:	43kDa

Synonyms:

MRPS9, MRP-S9, RPMS9, S9mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein.

UniProt Protein Function:	MRPS9: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Mitochondrial; Ribosomal Chromosomal Location of Human Ortholog: 2q12.1 Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion; nucleolus Molecular Function:protein binding; structural constituent of ribosome Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); translation
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P82933
NCBI GenInfo Identifier:	218511769
NCBI Gene ID:	64965
NCBI Accession:	P82933.2
UniProt Secondary Accession:	P82933,Q6PG40,
UniProt Related Accession:	P82933
Molecular Weight:	45,835 Da
NCBI Full Name:	28S ribosomal protein S9, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S9
NCBI Official Symbol:	MRPS9
NCBI Official Synonym Symbols:	S9mt; RPMS9; MRP-S9
NCBI Protein Information:	28S ribosomal protein S9, mitochondrial
UniProt Protein Name:	28S ribosomal protein S9, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS9
UniProt Entry Name:	RT09_HUMAN

Western blot analysis of extracts of various cell lines, using MRPS9 antibody (CAB11729) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

MRPS9 Antibody (PACO01110)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)