Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin

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SKU:
IVMB0144
Product Type:
In Vivo Monoclonal Antibody
Clone:
55R-170
Protein:
TNFR1
Isotype:
Armenian Hamster IgG
Reactivity:
Mouse
Synonyms:
TNFR-I
TNFRSF1A
P55
P60
Research Area:
Immune Checkpoint & Cancer Biology
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Armenian Hamster
Applications:
Blocking
FC
IP
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0144
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:55R-170
Protein:TNFR1
Product Type:Monoclonal Antibody
Synonyms:TNFR-I, TNFRSF1A, P55, P60
Isotype:Armenian Hamster IgG
Reactivity:Mouse
Immunogen:Purified Recombinant Mouse TNFR1 (>98%)
Applications:B, FC, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0142Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Low EndotoxinLow
IVMB0144Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, FC, IP, WB
Recommended Usage:eLISA
This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
WB To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents and a chromogenic substrate, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
FC
Reactivity:Mouse
Host Species:Armenian Hamster
Specificity:Clone 55R-170 recognizes mouse CD120a.
Antigen Distribution:CD120a is constitutively expressed in most tissues.
Immunogen:Purified Recombinant Mouse TNFR1 (>98%)
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-α and TNF-β (also known as LT-α). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein). Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Technical Datasheet:View
Protein:TNFR1
Function:Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor:TNF-α, LT-α (TNF-β)
Research Area:Immunology, Innate Immunity
Product Thumbnail
Armenian Hamster IgG Isotype Control
Clone PIP
Isotype Armenian Hamster IgG1
Endotoxin Level Ultra Low Endotoxin
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