Anti-MMP8 Antibody (CAB1963)
- SKU:
- CAB1963
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MMP8 Antibody |
| Antibody SKU: | CAB1963 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 101-467 of human MMP8 (NP_002415.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HL-60, HepG2, Jurkat, MCF7, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 101-467 of human MMP8 (NP_002415.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LTPG NPKW ERTN LTYR IRNY TPQL SEAE VERA IKDA FELW SVAS PLIF TRIS QGEA DINI AFYQ RDHG DNSP FDGP NGIL AHAF QPGQ GIGG DAHF DAEE TWTN TSAN YNLF LVAA HEFG HSLG LAHS SDPG ALMY PNYA FRET SNYS LPQD DIDG IQAI YGLS SNPI QPTG PSTP KPCD PSLT FDAI TTLR GEIL FFKD RYFW RRHP QLQR VEMN FISL FWPS LPTG IQAA YEDF DRDL IFLF KGNQ YWAL SGYD ILQG YPKD ISNY GFPS SVQA IDAA VFYR SKTY FFVN DQFW RYDN QRQF MEPG YPKS ISGA FPGI ESKV DAVF QQEH FFHV FSGP RYYA FDLI AQRV TRVA RGNK WLNC RYG |
| Gene ID: | 4317 |
| Uniprot: | P22894 |
| Cellular Location: | Cytoplasmic granule, Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 53kDa |
| Observed MW: | 65kDa |
| Synonyms: | MMP8, CLG1, HNC, MMP-8, PMNL-CL |
| Background: | This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.24.34; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region Molecular Function:zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22894 |
| NCBI GenInfo Identifier: | 116862 |
| NCBI Gene ID: | 4317 |
| NCBI Accession: | P22894.1 |
| UniProt Secondary Accession: | P22894,Q45F99, |
| UniProt Related Accession: | P22894 |
| Molecular Weight: | 53,412 Da |
| NCBI Full Name: | Neutrophil collagenase |
| NCBI Synonym Full Names: | matrix metallopeptidase 8 (neutrophil collagenase) |
| NCBI Official Symbol: | MMP8 |
| NCBI Official Synonym Symbols: | HNC; CLG1; MMP-8; PMNL-CL |
| NCBI Protein Information: | neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase) |
| UniProt Protein Name: | Neutrophil collagenase |
| UniProt Synonym Protein Names: | Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL |
| Protein Family: | Neutrophil collagenase |
| UniProt Gene Name: | MMP8 |
| UniProt Entry Name: | MMP8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MMP8 antibody (CAB1963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using MMP8 Rabbit pAb (CAB1963) at dilution of 1:150 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using MMP8 antibody (CAB1963) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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