Anti-MGAT1 항체 (CAB6387)

Antibody Name:	Anti-MGAT1 Antibody
Antibody SKU:	CAB6387
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GSAV THIR QPDL SSIA VPPD HRKF QGYY KIAR HYRW ALGQ VFRQ FRFP AAVV VEDD LEVA PDFF EYFR ATYP LLKA DPSL WCVS AWND NGKE QMVD ASRP ELLY RTDF FPGL GWLL LAEL WAEL EPKW PKAF WDDW MRRP EQRQ GRAC IRPE ISRT MTFG RKGV SHGQ FFDQ HLKF IKLN QQFV HFTQ LDLS YLQR EAYD RDFL ARVY GAPQ LQVE KVRT NDRK ELGE VRVQ YTGR DSFK AFAK ALGV MDDL KSGV PRAG YRGI VTFQ FRGR RVHL APPL TWEG YDPS WN
Gene ID:	4245
Uniprot:	P26572
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	50kDa
Observed MW:	60kDa

Synonyms:

MGAT1, GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, GnTI, MGAT, alpha-1

Background:

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1, 2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

UniProt Protein Function:	MGAT1: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Belongs to the glycosyltransferase 13 family.
UniProt Protein Details:	Protein type:EC 2.4.1.101; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Transferase Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: Golgi membrane; membrane Molecular Function:acetylglucosaminyltransferase activity; alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Biological Process: carbohydrate metabolic process
NCBI Summary:	There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P26572
NCBI GenInfo Identifier:	311033399
NCBI Gene ID:	4245
NCBI Accession:	P26572.2
UniProt Secondary Accession:	P26572,Q6IBE3, A8K404, B3KRU8, D3DWR1,
UniProt Related Accession:	P26572
Molecular Weight:	51kDa
NCBI Full Name:	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Synonym Full Names:	mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol:	MGAT1
NCBI Official Synonym Symbols:	GnTI; MGAT; GLCT1; GLYT1; GNT-1; GNT-I; GLCNAC-TI
NCBI Protein Information:	alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Protein Name:	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Synonym Protein Names:	N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I; GNT-I; GlcNAc-T I
UniProt Gene Name:	MGAT1

	Western blot analysis of extracts of various cell lines, using MGAT1 antibody (CAB6387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using MGAT1 antibody (CAB6387) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody (CAB6387) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using MGAT1 Rabbit pAb (CAB6387) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-MGAT1 Antibody (CAB12459)

MGAT1 Antibody (PACO10476)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)