Anti-LMNB2 Antibody (CAB6483)
- SKU:
- CAB6483
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-LMNB2 Antibody |
| Antibody SKU: | CAB6483 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human LMNB2 (NP_116126.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, MCF7, SW620, Mouse liver, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human LMNB2 (NP_116126.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AMAG ERDK FRKM LDAK EQEM TEMR DVMQ QQLA EYQE LLDV KLAL DMEI NAYR KLLE GEEE RLKL SPSP SSRV TVSR ATSS SSGS LSAT GRLG RSKR KRLE VEEP LGSG PSVL GTGT GGSG GFHL AQQA SASG SVSI EEID LEGK FVQL KNNS DKDQ SLGN WRIK RQVL EGEE IAYK FTPK YILR AGQM VTVW AAGA GVAH SPPS TLVW KGQS SWGT GESF RTVL VNAD GEEV AMRT VKKS SVMR ENEN GEEE EEEA EFGE EDLF HQQG DPRT TSRG CYVM |
| Gene ID: | 84823 |
| Uniprot: | Q03252 |
| Cellular Location: | Lipid-anchor, Nucleoplasmic side, Nucleus inner membrane |
| Calculated MW: | 69kDa |
| Observed MW: | 70kDa |
| Synonyms: | LMNB2, EPM9, LAMB2, LMN2, lamin-B2 |
| Background: | This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. |
| UniProt Protein Function: | Lamin B2: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Lamin B2 may be lost during the follicle centre stage of B-cell differentiation in humans. Nuclear lamins are cleaved by caspases during apoptosis. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: lamin filament; nuclear inner membrane Molecular Function:structural molecule activity Disease: Lipodystrophy, Partial, Acquired, Susceptibility To |
| UniProt Code: | Q03252 |
| NCBI GenInfo Identifier: | 23503078 |
| NCBI Gene ID: | |
| NCBI Accession: | Q03252.3 |
| Molecular Weight: | |
| NCBI Full Name: | Lamin-B2 |
| UniProt Protein Name: | Lamin-B2 |
| Protein Family: | Lamin |
| UniProt Gene Name: | LMNB2 |
| UniProt Entry Name: | LMNB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LMNB2 antibody (CAB6483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody (CAB6483) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human adenomyosis using LMNB2 Antibody (CAB6483) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody (CAB6483) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using LMNB2 antibody (CAB6483). Blue: DAPI for nuclear staining. |
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