Anti-LDHA Antibody (CAB1146)

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SKU:
CAB1146
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-LDHA Antibody
Antibody SKU:CAB1146
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 293T, NIH/3T3, K-562, Jurkat, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATL KDQL IYNL LKEE QTPQ NKIT VVGV GAVG MACA ISIL MKDL ADEL ALVD VIED KLKG EMMD LQHG SLFL RTPK IVSG KDYN VTAN SKLV IITA GARQ QEGE SRLN LVQR NVNI FKFI IPNV VKYS PNCK LLIV SNPV DILT YVAW KISG FPKN RVIG SGCN LDSA RFRY LMGE RLGV HPLS CHGW VLGE HGDS SVPV WSGM NVAG VSLK TLHP DLGT DKDK EQWK EVHK QVVE SAYE VIKL KGYT SWAI GLSV ADLA ESIM KNLR RVHP VSTM IKGL YGIK DDVF LSVP CILG QNGI SDLV KVTL TSEE EARL KKSA DTLW GIQK ELQF
Gene ID:3939
Uniprot:P00338
Cellular Location:Cytoplasm
Calculated MW:26kDa/30kDa/36kDa/39kDa
Observed MW:37kDa
Synonyms:GSD11, HEL-S-133P, LDHM, PIG19, LDHA
Background:The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
NCBI Summary:The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
UniProt Code:P00338
NCBI GenInfo Identifier:126047
NCBI Gene ID:3939
NCBI Accession:P00338.2
UniProt Secondary Accession:P00338,Q53G53, Q6IBM7, Q6ZNV1, Q9UDE8, Q9UDE9, B4DKQ2 B7Z5E3, D3DQY3, F8W819,
UniProt Related Accession:P00338
Molecular Weight:26,712 Da
NCBI Full Name:L-lactate dehydrogenase A chain
NCBI Synonym Full Names:lactate dehydrogenase A
NCBI Official Symbol:LDHA  
NCBI Official Synonym Symbols:LDHM; GSD11; PIG19; HEL-S-133P  
NCBI Protein Information:L-lactate dehydrogenase A chain
UniProt Protein Name:L-lactate dehydrogenase A chain
UniProt Synonym Protein Names:Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:LDHA  
UniProt Entry Name:LDHA_HUMAN
Anti-LDHA Antibody (CAB1146)Western blot analysis of extracts of various cell lines, using LDHA antibody (CAB1146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-LDHA Antibody (CAB1146)Immunofluorescence analysis of A549 cells using LDHA antibody (CAB1146). Blue: DAPI for nuclear staining.

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