Anti-KYNU Antibody (CAB6643)
- SKU:
- CAB6643
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-KYNU Antibody |
| Antibody SKU: | CAB6643 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human KYNU (NP_001028170.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, THP-1, NCI-H460, HepG2, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human KYNU (NP_001028170.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEPS SLEL PADT VQRI AAEL KCHP TDER VALH LDEE DKLR HFRE CFYI PKIQ DLPP VDLS LVNK DENA IYFL GNSL GLQP KMVK TYLE EELD KWAK IAAY GHEV GKRP WITG DESI VGLM KDIV GANE KEIA LMNA LTVN LHLL MLSF FKPT PKRY KILL EAKA FPSD HYAI ESQL QLHG LNIE ESMR MIKP REGE ETLR IEDI LEVI EKEG DSIA VILF SGVH FYTG QHFN IPAI TKAG QAKG CYVG FDLA HAVG NVEL YLHD WGVD FACW CSYK YLNA GAGG IAGA FIHE KHAH TIKP ARSE FFN |
| Gene ID: | 8942 |
| Uniprot: | Q16719 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 34kDa/52kDa |
| Observed MW: | 52kDa |
| Synonyms: | KYNU, KYNUU |
| Background: | Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.7.1.3; Mitochondrial; Amino Acid Metabolism - tryptophan; Hydrolase Chromosomal Location of Human Ortholog: 2q22.2 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol Molecular Function:protein homodimerization activity; kynureninase activity; pyridoxal phosphate binding Biological Process: anthranilate metabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; quinolinate biosynthetic process; tryptophan catabolic process Disease: Hydroxykynureninuria |
| NCBI Summary: | Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] |
| UniProt Code: | Q16719 |
| NCBI GenInfo Identifier: | 3913982 |
| NCBI Gene ID: | 8942 |
| NCBI Accession: | Q16719.1 |
| UniProt Secondary Accession: | Q16719,Q6I9T2, Q9BVW3, B2RCZ5, D3DP79, |
| UniProt Related Accession: | Q16719 |
| Molecular Weight: | 34,635 Da |
| NCBI Full Name: | Kynureninase |
| NCBI Synonym Full Names: | kynureninase |
| NCBI Official Symbol: | KYNU |
| NCBI Protein Information: | kynureninase; KYNU; L-kynurenine hydrolase |
| UniProt Protein Name: | Kynureninase |
| UniProt Synonym Protein Names: | L-kynurenine hydrolase |
| UniProt Gene Name: | KYNU |
| UniProt Entry Name: | KYNU_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KYNU antibody (CAB6643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using KYNU antibody (CAB6643) at dilution of 1:100 (40x lens). |
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