Anti-ITGA4 Antibody (CAB0696)

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SKU:
CAB0696
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-ITGA4 Antibody
Antibody SKU:CAB0696
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human ITGA4 (NP_000876.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HL-60, HepG2, THP-1, Mouse lung, Mouse spleen, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human ITGA4 (NP_000876.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FLCN FGKM ESGK EASV HIQL EGRP SILE MDET SALK FEIR ATGF PEPN PRVI ELNK DENV AHVL LEGL HHQR PKRY FTIV IISS SLLL GLIV LLLI SYVM WKAG FFKR QYKS ILQE ENRR DSWS YINS KSND D
Gene ID:3676
Uniprot:P13612
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:21kDa/114kDa
Observed MW:80kDa/130kDa/160kDa/170kDa
Synonyms:ITGA4, CD49D, IA4
Background:The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: focal adhesion; cell surface; membrane; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function:protein binding; fibronectin binding; metal ion binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; heart development; cell-matrix adhesion; receptor clustering; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; B cell differentiation; blood vessel remodeling; blood coagulation; leukocyte tethering or rolling; leukocyte migration

NCBI Summary:The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:P13612
NCBI GenInfo Identifier:311033436
NCBI Gene ID:3676
NCBI Accession:P13612.3
UniProt Related Accession:P13612
Molecular Weight:
NCBI Full Name:Integrin alpha-4
NCBI Synonym Full Names:integrin subunit alpha 4
NCBI Official Symbol:ITGA4  
NCBI Official Synonym Symbols:IA4; CD49D  
NCBI Protein Information:integrin alpha-4
UniProt Protein Name:Integrin alpha-4
UniProt Synonym Protein Names:CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD_antigen: CD49d
Protein Family:Integrin
UniProt Gene Name:ITGA4  
UniProt Entry Name:ITA4_HUMAN
Anti-ITGA4 Antibody (CAB0696)Western blot analysis of extracts of various cell lines, using ITGA4 antibody (CAB0696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ITGA4 Antibody (CAB0696)Western blot analysis of extracts of various cell lines, using ITGA4 antibody (CAB0696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

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