Anti-IPO8 항체 (CAB14679)

Antibody Name:	Anti-IPO8 Antibody
Antibody SKU:	CAB14679
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 878-1037 of human IPO8 (NP_006381.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, Mouse brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 878-1037 of human IPO8 (NP_006381.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TRQL VNRE DRSK AEKA DMEE NEEI SSDE EETN VTAQ AMQS NNGR GEDE EEED DDWD EEVL EETA LEGF STPL DLDN SVDE YQFF TQAL ITVQ SRDA AWYQ LLMA PLSE DQRT ALQE VYTL AEHR RTVA EAKK KIEQ QGGF TFEN KGVL SAFN FGTV PSNN
Gene ID:	10526
Uniprot:	O15397
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	96kDa/119kDa
Observed MW:	120kDa

Synonyms:

IPO8, RANBP8

Background:

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	IPO8: Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter- like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Karyopherin; Nuclear import; G protein regulator, misc.; Nuclear export Chromosomal Location of Human Ortholog: 12p11.21 Cellular Component: nucleoplasm; cytosol Molecular Function:protein binding; Ran GTPase binding Biological Process: intracellular protein transport; gene expression; signal transduction
NCBI Summary:	The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	O15397
NCBI GenInfo Identifier:	300360505
NCBI Gene ID:	10526
NCBI Accession:	NP_001177924.1
UniProt Secondary Accession:	O15397,B7Z7M3,
UniProt Related Accession:	O15397
Molecular Weight:	96,036 Da
NCBI Full Name:	importin-8 isoform 2
NCBI Synonym Full Names:	importin 8
NCBI Official Symbol:	IPO8
NCBI Official Synonym Symbols:	RANBP8
NCBI Protein Information:	importin-8
UniProt Protein Name:	Importin-8
UniProt Synonym Protein Names:	Ran-binding protein 8; RanBP8
Protein Family:	Importin
UniProt Gene Name:	IPO8
UniProt Entry Name:	IPO8_HUMAN

	Western blot analysis of extracts of various cell lines, using IPO8 antibody (CAB14679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using IPO8 antibody (CAB14679) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using IPO8 antibody (CAB14679) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using IPO8 antibody (CAB14679) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)