Anti-IGF1R Antibody (CAB14698)

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SKU:
CAB14698
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-IGF1R Antibody
Antibody SKU:CAB14698
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 741-910 of human IGF1R (NP_000866.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 741-910 of human IGF1R (NP_000866.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DVMQ VANT TMSS RSRN TTAA DTYN ITDP EELE TEYP FFES RVDN KERT VISN LRPF TLYR IDIH SCNH EAEK LGCS ASNF VFAR TMPA EGAD DIPG PVTW EPRP ENSI FLKW PEPE NPNG LILM YEIK YGSQ VEDQ RECV SRQE YRKY GGAK LNRL NPGN YTAR IQAT SL
Gene ID:3480
Uniprot:P08069
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:154kDa
Observed MW:110kDa
Synonyms:IGF1R, CD221, IGFIR, IGFR, JTK13
Background:This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.
UniProt Protein Details:

Protein type:Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: integral to plasma membrane; intracellular membrane-bound organelle; membrane; plasma membrane; receptor complex

Molecular Function:ATP binding; identical protein binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor receptor activity; phosphoinositide 3-kinase binding; protein binding; protein-tyrosine kinase activity

Biological Process: brain development; epidermis development; exocrine pancreas development; immune response; inactivation of MAPKK activity; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; male sex determination; mammary gland development; negative regulation of apoptosis; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; protein tetramerization; regulation of JNK cascade; signal transduction

Disease: Insulin-like Growth Factor I, Resistance To

NCBI Summary:This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Code:P08069
NCBI GenInfo Identifier:124240
NCBI Gene ID:3480
NCBI Accession:P08069.1
UniProt Secondary Accession:P08069,Q14CV2, Q9UCC0, B1B5Y2,
UniProt Related Accession:P08069
Molecular Weight:
NCBI Full Name:Insulin-like growth factor 1 receptor
NCBI Synonym Full Names:insulin like growth factor 1 receptor
NCBI Official Symbol:IGF1R  
NCBI Official Synonym Symbols:IGFR; CD221; IGFIR; JTK13  
NCBI Protein Information:insulin-like growth factor 1 receptor
UniProt Protein Name:Insulin-like growth factor 1 receptor
UniProt Synonym Protein Names:Insulin-like growth factor I receptor; IGF-I receptor
Protein Family:Insulin-like growth factor 1 receptor
UniProt Gene Name:IGF1R  
UniProt Entry Name:IGF1R_HUMAN
Anti-IGF1R Antibody (CAB14698)Western blot analysis of extracts of various cell lines, using IGF1R antibody (CAB14698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

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