Anti-IDH1 Antibody (CAB13245)[KO Validated]

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SKU:
CAB13245
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-IDH1 Antibody [KO Validated]
Antibody SKU:CAB13245
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSKK ISGG SVVE MQGD EMTR IIWE LIKE KLIF PYVE LDLH SYDL GIEN RDAT NDQV TKDA AEAI KKHN VGVK CATI TPDE KRVE EFKL KQMW KSPN GTIR NILG GTVF REAI ICKN IPRL VSGW VKPI IIGR HAYG DQYR ATDF VVPG PGKV EITY TPSD GTQK VTYL VHNF EEGG GVAM GMYN QDKS IEDF AHSS FQMA LSKG WPLY LSTK NTIL KKYD GRFK DIFQ EIYD KQYK SQFE AQKI WYEH RLID DMVA QAMK SEGG FIWA CKNY DGDV QSDS VAQG YGSL GMMT SVLV CPDG KTVE AEAA HGTV TRHY RMYQ KGQE TSTN PIAS IFAW TRGL AHRA KLDN NKEL AFFA NALE EVSI ETIE AGFM TKDL AACI KGLP NVQR SDYL NTFE FMDK LGEN LKIK LAQA KL
Gene ID:3417
Uniprot:O75874
Cellular Location:Cytoplasm, Peroxisome
Calculated MW:46kDa
Observed MW:46KDa
Synonyms:IDH1, HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
Background:Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; peroxisomal matrix; peroxisome

Molecular Function:(R)-2-hydroxyglutarate dehydrogenase activity; isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; protein homodimerization activity; receptor binding

Biological Process: 2-oxoglutarate metabolic process; isocitrate metabolic process; NADPH regeneration

Disease: Glioma Susceptibility 1

NCBI Summary:Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
UniProt Code:O75874
NCBI GenInfo Identifier:21903432
NCBI Gene ID:3417
NCBI Accession:O75874.2
UniProt Secondary Accession:O75874,Q567U4, Q6FHQ6, Q7Z3V0, Q93090, Q9NTJ9, Q9UKW8
UniProt Related Accession:O75874
Molecular Weight:46,659 Da
NCBI Full Name:Isocitrate dehydrogenase
NCBI Synonym Full Names:isocitrate dehydrogenase (NADP(+)) 1, cytosolic
NCBI Official Symbol:IDH1  
NCBI Official Synonym Symbols:IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26  
NCBI Protein Information:isocitrate dehydrogenase [NADP] cytoplasmic
UniProt Protein Name:Isocitrate dehydrogenase [NADP] cytoplasmic
UniProt Synonym Protein Names:Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Protein Family:Isocitrate dehydrogenase
UniProt Gene Name:IDH1  
Anti-IDH1 Antibody [KO Validated] (CAB13245)Western blot analysis of extracts of 293T cells, using IDH1 antibody (CAB13245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-IDH1 Antibody [KO Validated] (CAB13245)Immunofluorescence analysis of C6 cells using IDH1 Polyclonal Antibody (CAB13245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-IDH1 Antibody [KO Validated] (CAB13245)Immunofluorescence analysis of L929 cells using IDH1 Polyclonal Antibody (CAB13245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-IDH1 Antibody [KO Validated] (CAB13245)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IDH1 antibody (CAB13245). Western blot was performed from the immunoprecipitate using IDH1 antibody (CAB13245) at a dilition of 1:1000.

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