Anti-HBEGF 항체 (CAB1695)

Antibody Name:	Anti-HBEGF Antibody
Antibody SKU:	CAB1695
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human HBEGF (NP_001936.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human HBEGF (NP_001936.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVTG ESLE RLRR GLAA GTSN PDPP TVST DQLL PLGG GRDR KVRD LQEA DLDL LRVT LSSK PQAL ATPN KEEH GKRK KKGK GLGK KRDP CLRK YKDF CIHG ECKY VKEL RAPS CICH PGYH GERC HGLS LPVE NRLY TYDH T
Gene ID:	1839
Uniprot:	Q99075
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein, extracellular space
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:	HBEGF, DTR, DTS, DTSF, HEGFL
Background:

UniProt Protein Function:	HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q23 Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; plasma membrane Molecular Function:heparin binding; growth factor activity; epidermal growth factor receptor binding Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; positive regulation of cell migration; wound healing, spreading of epidermal cells
UniProt Code:	Q99075
NCBI GenInfo Identifier:	544477
NCBI Gene ID:	1839
NCBI Accession:	Q99075.1
UniProt Secondary Accession:	Q99075,B2R821,
UniProt Related Accession:	Q99075
Molecular Weight:	Calculated MW: 23kDaObserved MW: 23kDa
NCBI Full Name:	Proheparin-binding EGF-like growth factor
NCBI Synonym Full Names:	heparin-binding EGF-like growth factor
NCBI Official Symbol:	HBEGF
NCBI Official Synonym Symbols:	DTR; DTS; DTSF; HEGFL
NCBI Protein Information:	proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
UniProt Protein Name:	Proheparin-binding EGF-like growth factor
UniProt Synonym Protein Names:	Diphtheria toxin receptor; DT-R
Protein Family:	Proheparin-binding EGF-like growth factor
UniProt Gene Name:	HBEGF
UniProt Entry Name:	HBEGF_HUMAN

Western blot analysis of extracts of various cell lines, using HBEGF antibody (CAB1695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies	ELISA Kits
Anti-HBEGF Antibody (CAB16365)	Human Proheparin-binding EGF-like growth factor (HBEGF) ELISA Kit
Anti-HB EGF[KO Validated] Antibody (CAB11657)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)