Anti-GSTM4 항체 (CAB7434)

Antibody Name:	Anti-GSTM4 Antibody
Antibody SKU:	CAB7434
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human GSTM4 (NP_000841.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SW480, NCI-H460, Mouse liver, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human GSTM4 (NP_000841.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSMT LGYW DIRG LAHA IRLL LEYT DSSY EEKK YTMG DAPD YDRS QWLN EKFK LGLD FPNL PYLI DGAH KITQ SNAI LCYI ARKH NLCG ETEE EKIR VDIL ENQA MDVS NQLA RVCY SPDF EKLK PEYL EELP TMMQ HFSQ FLGK RPWF VGDK ITFV DFLA YDVL DLHR IFEP NCLD AFPN LKDF ISRF EGLE KISA YMKS SRFL PKPL YTRV AVWG NK
Gene ID:	2948
Uniprot:	Q03013
Cellular Location:	Cytoplasm
Calculated MW:	18kDa/22kDa/25kDa
Observed MW:	26-34kDa

Synonyms:

GSTM4, GSTM4-4, GTM4

Background:

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. Multiple transcript variants, each encoding a distinct protein isoform, have been identified.

UniProt Protein Function:	GSTM4: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1- chloro-2,4-dinitrobenzene. Belongs to the GST superfamily. Mu family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytoplasm; cytosol Molecular Function:enzyme binding; glutathione binding; glutathione transferase activity; protein binding; protein homodimerization activity Biological Process: glutathione metabolic process; xenobiotic catabolic process
NCBI Summary:	Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. Multiple transcript variants, each encoding a distinct protein isoform, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q03013
NCBI GenInfo Identifier:	1170096
NCBI Gene ID:	2948
NCBI Accession:	Q03013.3
UniProt Secondary Accession:	Q03013,Q05465, Q32NC1, Q4JNT8, Q6FH87, A8K765,
UniProt Related Accession:	Q03013
Molecular Weight:	18,627 Da
NCBI Full Name:	Glutathione S-transferase Mu 4
NCBI Synonym Full Names:	glutathione S-transferase mu 4
NCBI Official Symbol:	GSTM4
NCBI Official Synonym Symbols:	GTM4; GSTM4-4
NCBI Protein Information:	glutathione S-transferase Mu 4
UniProt Protein Name:	Glutathione S-transferase Mu 4
UniProt Synonym Protein Names:	GST class-mu 4; GST-Mu2; GSTM4-4
Protein Family:	Glutathione S-transferase
UniProt Gene Name:	GSTM4
UniProt Entry Name:	GSTM4_HUMAN

Western blot analysis of extracts of various cell lines, using GSTM4 antibody (CAB7434) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

Antibodies

GSTM4 Antibody (PACO09617)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)