Anti-GGA2 Antibody (CAB1186)
- SKU:
- CAB1186
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GGA2 Antibody |
| Antibody SKU: | CAB1186 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, Raji, MCF7, THP-1, 22Rv1, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAT AVAA AVAG TESA QGPP GPAA SLEL WLNK ATDP SMSE QDWS AIQN FCEQ VNTD PNGP THAP WLLA HKIQ SPQE KEAL YALT VLEM CMNH CGEK FHSE VAKF RFLN ELIK VLSP KYLG SWAT GKVK GRVI EILF SWTV WFPE DIKI RDAY QMLK KQGI IKQD PKLP VDKI LPPP SPWP KSSI FDAD EEKS KLLT RLLK SNHP EDLQ AANR LIKN LVKE EQEK SEKV SKRV SAVE EVRS HVKV LQEM LSMY RRPG QAPP DQEA LQVV YERC EKLR PTLF RLAS DTTD DDDA LAEI LQAN |
| Gene ID: | 23062 |
| Uniprot: | Q9UJY4 |
| Cellular Location: | Endosome membrane, Golgi apparatus, Peripheral membrane protein, trans-Golgi network membrane |
| Calculated MW: | 67kDa |
| Observed MW: | 72-80kDa |
| Synonyms: | GGA2, VEAR |
| Background: | This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. |
| UniProt Protein Function: | GGA2: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Vesicle Chromosomal Location of Human Ortholog: 16p12 Cellular Component: Golgi apparatus; clathrin adaptor complex; clathrin-coated vesicle; endosome membrane; trans-Golgi network Molecular Function:ADP-ribosylation factor binding; protein binding Biological Process: vesicle-mediated transport; intracellular protein transport |
| NCBI Summary: | This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UJY4 |
| NCBI GenInfo Identifier: | 143811397 |
| NCBI Gene ID: | 23062 |
| NCBI Accession: | Q9UJY4.3 |
| UniProt Related Accession: | Q9UJY4 |
| Molecular Weight: | ~ 72-80kDa |
| NCBI Full Name: | ADP-ribosylation factor-binding protein GGA2 |
| NCBI Synonym Full Names: | golgi associated, gamma adaptin ear containing, ARF binding protein 2 |
| NCBI Official Symbol: | GGA2 |
| NCBI Official Synonym Symbols: | VEAR |
| NCBI Protein Information: | ADP-ribosylation factor-binding protein GGA2 |
| UniProt Protein Name: | ADP-ribosylation factor-binding protein GGA2 |
| UniProt Synonym Protein Names: | Gamma-adaptin-related protein 2; Golgi-localized, gamma ear-containing, ARF-binding protein 2; VHS domain and ear domain of gamma-adaptin; Vear |
| Protein Family: | ADP-ribosylation factor-binding protein |
| UniProt Gene Name: | GGA2 |
| UniProt Entry Name: | GGA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GGA2 antibody (CAB1186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody (CAB1186) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody (CAB1186) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody (CAB1186) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using GGA2 antibody (CAB1186). Blue: DAPI for nuclear staining. |
Related Products
