Anti-ERC1 항체 (CAB9509)

Antibody Name:	Anti-ERC1 Antibody
Antibody SKU:	CAB9509
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 700-850 of human ERC1 (NP_829884.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, NIH/3T3, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 700-850 of human ERC1 (NP_829884.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EIAL EQKK EECL KMES QLKK AHEA ALEA RASP EMSD RIQH LERE ITRY KDES SKAQ AEVD RLLE ILKE VENE KNDK DKKI AELE RQVK DQNK KVAN LKHK EQVE KKKS AQML EEAR RRED NLND SSQQ LQDS LRKK DDRI EELE EALR ESV
Gene ID:	23085
Uniprot:	Q8IUD2
Cellular Location:	Cytoplasm, Golgi apparatus membrane, Membrane, Peripheral membrane protein
Calculated MW:	81kDa/108kDa/113kDa/124kDa/128kDa
Observed MW:	130 120kDa

Synonyms:	ERC1, Cast2, ELKS, ERC-1, RAB6IP2
Background:	The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13) in thyroid papillary carcinoma. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ERC1: Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA. Isoform 4 interacts with PPFIA1, and through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1. Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1. Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: presynaptic membrane; Golgi membrane; cytoplasm; synapse; IkappaB kinase complex Molecular Function:protein binding; leucine zipper domain binding; Rab GTPase binding; PDZ domain binding Biological Process: protein transport; regulation of transcription, DNA-dependent; multicellular organismal development; I-kappaB phosphorylation; retrograde transport, endosome to Golgi; activation of NF-kappaB transcription factor
NCBI Summary:	The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IUD2
NCBI GenInfo Identifier:	38045898
NCBI Gene ID:	23085
NCBI Accession:	NP_829884.1
UniProt Secondary Accession:	Q8IUD2,Q6NVK2, Q8IUD3, Q8IUD4, Q8IUD5, Q8NAS1, Q9NXN5 Q9UIK7, A2RU77, A7E295, D3DUP7, D3DUP8,
UniProt Related Accession:	Q8IUD2
Molecular Weight:	128,086 Da
NCBI Full Name:	ELKS/Rab6-interacting/CAST family member 1 isoform epsilon
NCBI Synonym Full Names:	ELKS/RAB6-interacting/CAST family member 1
NCBI Official Symbol:	ERC1
NCBI Official Synonym Symbols:	ELKS; Cast2; RAB6IP2
NCBI Protein Information:	ELKS/Rab6-interacting/CAST family member 1; RAB6 interacting protein 2; Rab6-interacting protein 2
UniProt Protein Name:	ELKS/Rab6-interacting/CAST family member 1
UniProt Synonym Protein Names:	Rab6-interacting protein 2
Protein Family:	ELKS/Rab6-interacting/CAST family
UniProt Gene Name:	ERC1
UniProt Entry Name:	RB6I2_HUMAN

Western blot analysis of extracts of various cell lines, using ERC1 antibody (CAB9509) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

ERC1 Antibody (PACO09125)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)