Anti-EEF1G Antibody (CAB7891)
- SKU:
- CAB7891
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EEF1G Antibody |
| Antibody SKU: | CAB7891 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human EEF1G (NP_001395.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BxPC-3, NIH/3T3, HeLa, Mouse brain, Mouse thymus, Mouse pancreas, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human EEF1G (NP_001395.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLWL YKQV LEPS FRQA FPNT NRWF LTCI NQPQ FRAV LGEV KLCE KMAQ FDAK KFAE TQPK KDTP RKEK GSRE EKQK PQAE RKEE KKAA APAP EEEM DECE QALA AEPK AKDP FAHL PKST FVLD EFKR KYSN EDTL SVAL PYFW EHFD KDGW SLWY SEYR FPEE LTQT FMSC NLIT GMFQ RLDK LRKN AFAS VILF GTNN SSSI SGVW VFRG QELA FPLS PDWQ VDYE SYTW RKLD PGSE ETQT LVRE YFSW EGAF QHVG KAFN QGKI FK |
| Gene ID: | 1937 |
| Uniprot: | P26641 |
| Cellular Location: | |
| Calculated MW: | 50kDa/56kDa |
| Observed MW: | 50kDa |
| Synonyms: | EEF1G, EF1G, GIG35 |
| Background: | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. |
| UniProt Protein Function: | EEF1G: Probably plays a role in anchoring the complex to other cellular components. |
| UniProt Protein Details: | Protein type:Translation; Translation elongation Chromosomal Location of Human Ortholog: 11q12.3 Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; membrane; nucleus Molecular Function:cadherin binding; protein binding Biological Process: response to virus; translational elongation |
| NCBI Summary: | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P26641 |
| NCBI GenInfo Identifier: | 119165 |
| NCBI Gene ID: | 1937 |
| NCBI Accession: | P26641.3 |
| UniProt Secondary Accession: | P26641,Q6PJ62, Q6PK31, Q96CU2, Q9P196, B4DTG2, |
| UniProt Related Accession: | P26641 |
| Molecular Weight: | 50kDa |
| NCBI Full Name: | Elongation factor 1-gamma |
| NCBI Synonym Full Names: | eukaryotic translation elongation factor 1 gamma |
| NCBI Official Symbol: | EEF1G |
| NCBI Official Synonym Symbols: | EF1G; GIG35 |
| NCBI Protein Information: | elongation factor 1-gamma |
| UniProt Protein Name: | Elongation factor 1-gamma |
| UniProt Synonym Protein Names: | eEF-1B gamma |
| Protein Family: | Elongation factor |
| UniProt Gene Name: | EEF1G |
 | Western blot analysis of extracts of various cell lines, using EEF1G antibody (CAB7891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat brain using EEF1G Rabbit pAb (CAB7891) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using EEF1G Rabbit pAb (CAB7891) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using EEF1G Rabbit pAb (CAB7891) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of C6 cells using EEF1G Polyclonal Antibody (CAB7891) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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