Anti-DHRS2 Antibody (CAB6446)
- SKU:
- CAB6446
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DHRS2 Antibody |
| Antibody SKU: | CAB6446 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DHRS2 (NP_878912.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, SKOV3, MCF7, BT-474, 22Rv1, Mouse liver, Mouse heart, Mouse bone marrow, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DHRS2 (NP_878912.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLSA VARG YQGW FHPC ARLS VRMS STGI DRKG VLAN RVAV VTGS TSGI GFAI ARRL ARDG AHVV ISSR KQQN VDRA MAKL QGEG LSVA GIVC HVGK AEDR EQLV AKAL EHCG GVDF LVCS AGVN PLVG STLG TSEQ IWDK ILSV NVKS PALL LSQL LPYM ENRR GAVI LVSS IAAY NPVV ALGV YNVS KTAL LGLT RTLA LELA PKDI RVNC VVPG IIKT DFSK VVRI GFMG MSLS GRTS RNII SCRG LGSQ RTVQ ESCP SCAL QMPA TSTG RTLR WQAT PLGS ERSG GGCV AVVP GPGA |
| Gene ID: | 10202 |
| Uniprot: | Q13268 |
| Cellular Location: | Mitochondrion matrix, Nucleus |
| Calculated MW: | 29kDa/31kDa |
| Observed MW: | 30-35kDa |
| Synonyms: | DHRS2, HEP27, SDR25C1 |
| Background: | This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family, which has over 46, 000 members. Members of this family are enzymes that metabolize many different compounds, such as steroid hormones, prostaglandins, retinoids, lipids and xenobiotics. Alternative promoter use and alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | DHRS2: Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2- propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. May inhibit cell replication. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.1.-.-; Oxidoreductase; EC 1.1.1.- Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nuclear envelope; nucleus Molecular Function:carbonyl reductase (NADPH) activity Biological Process: negative regulation of cell proliferation; response to toxin; C21-steroid hormone metabolic process; myeloid dendritic cell differentiation; negative regulation of apoptosis |
| UniProt Code: | Q13268 |
| NCBI GenInfo Identifier: | 527504085 |
| NCBI Gene ID: | 10202 |
| NCBI Accession: | Q13268.4 |
| UniProt Secondary Accession: | Q13268,Q53GS4, Q7Z789, Q9H2R2, D3DS54, |
| UniProt Related Accession: | Q13268 |
| Molecular Weight: | 258 |
| NCBI Full Name: | Dehydrogenase/reductase SDR family member 2, mitochondrial |
| NCBI Synonym Full Names: | dehydrogenase/reductase (SDR family) member 2 |
| NCBI Official Symbol: | DHRS2 |
| NCBI Official Synonym Symbols: | HEP27; SDR25C1 |
| NCBI Protein Information: | dehydrogenase/reductase SDR family member 2, mitochondrial; protein D; dicarbonyl reductase HEP27; dehydrogenase/reductase member 2; short-chain alcohol dehydrogenase family member; short chain dehydrogenase/reductase family 25C, member 1 |
| UniProt Protein Name: | Dehydrogenase/reductase SDR family member 2, mitochondrial |
| UniProt Synonym Protein Names: | Dicarbonyl reductase HEP27; Protein D |
| Protein Family: | Dehydrogenase/reductase SDR family |
| UniProt Gene Name: | DHRS2 |
| UniProt Entry Name: | DHRS2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DHRS2 antibody (CAB6446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using DHRS2 antibody (CAB6446) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human uterine cancer using DHRS2 antibody (CAB6446) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using DHRS2 antibody (CAB6446) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using DHRS2 antibody (CAB6446). Blue: DAPI for nuclear staining. |
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