Anti-DFFB Antibody (CAB10110)
- SKU:
- CAB10110
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-DFFB Antibody |
| Antibody SKU: | CAB10110 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:1000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, K-562, SW620, Mouse spleen, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DEQA PQRQ RLLA DLLH NVSQ NIAA ETRA EDPP WFEG LESR FQSK SGYL RYSC ESRI RSYL REVS SYPS TVGA EAQE EFLR VLGS MCQR LRSM QYNG SYFD RGAK GGSR LCTP EGWF SCQG PFDM DSCL SRHS INPY SNRE SRIL FSTW NLDH IIEK KRTI IPTL VEAI KEQD GREV DWEY FYGL LFTS ENLK LVHI VCHK KTTH KLNC DPSR IYKP QTRL KRKQ PVRK RQ |
| Gene ID: | 1677 |
| Uniprot: | O76075 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 11kDa/12kDa/30kDa/39kDa |
| Observed MW: | 39kDa |
| Synonyms: | DFFB, CAD, CPAN, DFF-40, DFF2, DFF40 |
| Background: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. |
| UniProt Protein Function: | DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.-.-.-; Apoptosis; Deoxyribonuclease Chromosomal Location of Human Ortholog: 1p36.3 Cellular Component: cytosol; nuclear chromatin; nucleolus; nucleoplasm; nucleus Molecular Function:deoxyribonuclease activity; enzyme binding Biological Process: apoptotic chromosome condensation; DNA fragmentation during apoptosis |
| NCBI Summary: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013] |
| UniProt Code: | O76075 |
| NCBI GenInfo Identifier: | 9087148 |
| NCBI Gene ID: | 1677 |
| NCBI Accession: | O76075.1 |
| UniProt Secondary Accession: | O76075,O60521, Q5SR22, Q9BYI4, Q9BYI5, Q9BYI6, |
| UniProt Related Accession: | O76075 |
| Molecular Weight: | 11,482 Da |
| NCBI Full Name: | DNA fragmentation factor subunit beta |
| NCBI Synonym Full Names: | DNA fragmentation factor subunit beta |
| NCBI Official Symbol: | DFFB |
| NCBI Official Synonym Symbols: | CAD; CPAN; DFF2; DFF40; DFF-40 |
| NCBI Protein Information: | DNA fragmentation factor subunit beta |
| UniProt Protein Name: | DNA fragmentation factor subunit beta |
| UniProt Synonym Protein Names: | Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40 |
| Protein Family: | DNA fragmentation factor |
| UniProt Gene Name: | DFFB |
| UniProt Entry Name: | DFFB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DFFB antibody (CAB10110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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