Anti-DDX24 Antibody (CAB15481)
- SKU:
- CAB15481
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DDX24 Antibody |
| Antibody SKU: | CAB15481 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 510-859 of human DDX24 (NP_065147.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, LO2, Jurkat, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 510-859 of human DDX24 (NP_065147.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LTLV HQAP ARIL HKKH TKKM DKTA KLDL LMQK IGMR GKPK VIDL TRNE ATVE TLTE TKIH CETD EKDF YLYY FLMQ YPGR SLVF ANSI SCIK RLSG LLKV LDIM PLTL HACM HQKQ RLRN LEQF ARLE DCVL LATD VAAR GLDI PKVQ HVIH YQVP RTSE IYVH RSGR TARA TNEG LSLM LIGP EDVI NFKK IYKT LKKD EDIP LFPV QTKY MDVV KERI RLAR QIEK SEYR NFQA CLHN SWIE QAAA ALEI ELEE DMYK GGKA DQQE ERRR QKQM KVLK KELR HLLS QPLF TESQ KTKY PTQS GKPP LLVS APSK SESA LSCL SKQK KKKT KKPK EPQP EQPQ PSTS AN |
| Gene ID: | 57062 |
| Uniprot: | Q9GZR7 |
| Cellular Location: | |
| Calculated MW: | 90kDa/96kDa |
| Observed MW: | 120kDa |
| Synonyms: | DDX24 |
| Background: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level. |
| UniProt Protein Function: | DDX24: ATP-dependent RNA helicase (Potential). Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily. |
| UniProt Protein Details: | Protein type:Helicase; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 14q32 Cellular Component: membrane; nucleolus Molecular Function:ATP-dependent RNA helicase activity Biological Process: RNA secondary structure unwinding |
| NCBI Summary: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9GZR7 |
| NCBI GenInfo Identifier: | 18202929 |
| NCBI Gene ID: | 57062 |
| NCBI Accession: | Q9GZR7.1 |
| UniProt Secondary Accession: | Q9GZR7,Q4V9L5, E7EMJ4, |
| UniProt Related Accession: | Q9GZR7 |
| Molecular Weight: | 90,298 Da |
| NCBI Full Name: | ATP-dependent RNA helicase DDX24 |
| NCBI Synonym Full Names: | DEAD-box helicase 24 |
| NCBI Official Symbol: | DDX24 |
| NCBI Protein Information: | ATP-dependent RNA helicase DDX24 |
| UniProt Protein Name: | ATP-dependent RNA helicase DDX24 |
| UniProt Synonym Protein Names: | DEAD box protein 24 |
| Protein Family: | ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX24 |
| UniProt Entry Name: | DDX24_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDX24 antibody (CAB15481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using DDX24 antibody (CAB15481) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using DDX24 antibody (CAB15481) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using DDX24 antibody (CAB15481) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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