Anti-DAPK3 Antibody (CAB15047)
- SKU:
- CAB15047
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-DAPK3 Antibody |
| Antibody SKU: | CAB15047 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAPK3 (NP_001339.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | mouse brain, mouse lung, rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAPK3 (NP_001339.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSTF RQED VEDH YEMG EELG SGQF AIVR KCRQ KGTG KEYA AKFI KKRR LSSS RRGV SREE IERE VNIL REIR HPNI ITLH DIFE NKTD VVLI LELV SGGE |
| Gene ID: | 1613 |
| Uniprot: | O43293 |
| Cellular Location: | Cytoplasm, Cytoplasm, Nucleus |
| Calculated MW: | 37kDa/52kDa |
| Observed MW: | 55kDa |
| Synonyms: | DAPK3, DLK, ZIP, ZIPK |
| Background: | Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. |
| UniProt Protein Function: | DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; DAPK family Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: PML body; cytoplasm; nucleus; lipid raft Molecular Function:identical protein binding; protein serine/threonine kinase activity; leucine zipper domain binding; protein binding; protein homodimerization activity; Rho GTPase binding; calmodulin-dependent protein kinase activity; cAMP response element binding protein binding; ATP binding Biological Process: regulation of smooth muscle contraction; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; regulation of myosin II filament assembly or disassembly; regulation of mitotic cell cycle; cytokinesis; chromatin modification; protein amino acid phosphorylation; regulation of apoptosis; neuron differentiation; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; regulation of mitosis; regulation of autophagy; positive regulation of cell migration |
| NCBI Summary: | Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43293 |
| NCBI GenInfo Identifier: | 4557511 |
| NCBI Gene ID: | 1613 |
| NCBI Accession: | NP_001339.1 |
| UniProt Related Accession: | O43293 |
| Molecular Weight: | ~ 52kDa |
| NCBI Full Name: | death-associated protein kinase 3 |
| NCBI Synonym Full Names: | death associated protein kinase 3 |
| NCBI Official Symbol: | DAPK3 |
| NCBI Official Synonym Symbols: | DLK; ZIP; ZIPK |
| NCBI Protein Information: | death-associated protein kinase 3 |
| UniProt Protein Name: | Death-associated protein kinase 3 |
| UniProt Synonym Protein Names: | DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase |
| Protein Family: | Myosin |
| UniProt Gene Name: | DAPK3 |
| UniProt Entry Name: | DAPK3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DAPK3 antibody (CAB15047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using DAPK3 antibody (CAB15047) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using DAPK3 antibody (CAB15047) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using DAPK3 antibody (CAB15047) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using DAPK3 antibody (CAB15047) at dilution of 1:100 (40x lens). |
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