Anti-CYP2E1 Antibody (CAB2160)
- SKU:
- CAB2160
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-CYP2E1 Antibody |
| Antibody SKU: | CAB2160 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KVIK NVAE VKEY VSER VKEH HQSL DPNC PRDL TDCL LVEM EKEK HSAE RLYT MDGI TVTV ADLF FAGT ETTS TTLR YGLL ILMK YPEI EEKL HEEI DRVI GPSR IPAI KDRQ EMPY MDAV VHEI QRFI TLVP SNLP HEAT RDTI FRGY LIPK GTVV VPTL DSVL YDNQ EFPD PEKF KPEH FLNE NGKF KYSD YFKP FSTG KRVC AGEG LARM ELFL LLCA ILQH FNLK PLVD PKDI DLSP IHIG FGCI PPRY KLCV IPRS |
| Gene ID: | 1571 |
| Uniprot: | P05181 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein |
| Calculated MW: | 56kDa |
| Observed MW: | 55kDa |
| Synonyms: | CYP2E1, CPE1, CYP2E, P450-J, P450C2E |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. |
| UniProt Protein Function: | CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.13.n7; Lipid Metabolism - arachidonic acid; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Mitochondrial Chromosomal Location of Human Ortholog: 10q26.3 Cellular Component: endoplasmic reticulum membrane Molecular Function:arachidonic acid epoxygenase activity; enzyme binding; heme binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; oxygen binding; steroid hydroxylase activity Biological Process: benzene metabolic process; carbon tetrachloride metabolic process; drug metabolic process; epoxygenase P450 pathway; halogenated hydrocarbon metabolic process; heterocycle metabolic process; monoterpenoid metabolic process; steroid metabolic process; xenobiotic metabolic process |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05181 |
| NCBI GenInfo Identifier: | 117250 |
| NCBI Gene ID: | 1571 |
| NCBI Accession: | P05181.1 |
| UniProt Secondary Accession: | P05181,Q5VZD5, Q6NWT9, Q9UK47, |
| UniProt Related Accession: | P05181 |
| Molecular Weight: | 56,849 Da |
| NCBI Full Name: | Cytochrome P450 2E1 |
| NCBI Synonym Full Names: | cytochrome P450 family 2 subfamily E member 1 |
| NCBI Official Symbol: | CYP2E1 |
| NCBI Official Synonym Symbols: | CPE1; CYP2E; P450-J; P450C2E |
| NCBI Protein Information: | cytochrome P450 2E1 |
| UniProt Protein Name: | Cytochrome P450 2E1 |
| UniProt Synonym Protein Names: | 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-J |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYP2E1 |
| UniProt Entry Name: | CP2E1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP2E1 antibody (CAB2160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody (CAB2160) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody (CAB2160) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using CYP2E1 antibody (CAB2160). Blue: DAPI for nuclear staining. |
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