Anti-CRYZ Antibody (CAB19997)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB19997
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-CRYZ Antibody [KO Validated]
Antibody SKU:CAB19997
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CRYZ.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human CRYZ.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1429
Uniprot:Q08257
Cellular Location:Cytoplasm
Calculated MW:20kDa/31kDa/35kDa
Observed MW:35KDa
Anti-CRYZ Antibody [KO Validated] (CAB19997)Western blot analysis of extracts from normal (control) and CRYZ knockout (KO) HeLa cells, using CRYZ antibody (CAB19997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:CRYZ
Background:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist.
UniProt Protein Function:CRYZ: Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.6.5.5; Oxidoreductase; RNA-binding

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: cytoplasm; cytosol

Molecular Function:mRNA 3'-UTR binding; NADPH:quinone reductase activity

Biological Process: protein homotetramerization; visual perception; xenobiotic catabolic process

NCBI Summary:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist. [provided by RefSeq, Sep 2008]
UniProt Code:Q08257
NCBI GenInfo Identifier:585013
NCBI Gene ID:1429
NCBI Accession:Q08257.1
UniProt Secondary Accession:Q08257,Q53FT0, Q59EU7, Q5HYE7, Q6NSK9, A6NN60, D3DQ76
UniProt Related Accession:Q08257
Molecular Weight:31,528 Da
NCBI Full Name:Quinone oxidoreductase
NCBI Synonym Full Names:crystallin zeta
NCBI Official Symbol:CRYZ  
NCBI Protein Information:quinone oxidoreductase
UniProt Protein Name:Quinone oxidoreductase
UniProt Synonym Protein Names:NADPH:quinone reductase; Zeta-crystallin
Protein Family:Zeta-crystallin
UniProt Gene Name:CRYZ  
UniProt Entry Name:QOR_HUMAN

0 Reviews

View AllClose

Related Products