Anti-CRKL Antibody (CAB0511)

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SKU:
CAB0511
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-CRKL Antibody
Antibody SKU:CAB0511
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide of human CRKL
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:MCF7, HeLa, Raji, Mouse brain, Mouse thymus, Mouse liver
Immunogen:A synthetic peptide of human CRKL
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1399
Uniprot:P46109
Cellular Location:
Calculated MW:33kDa
Observed MW:34kDa
Synonyms:CRKL
Background:This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
UniProt Protein Function:CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity

Biological Process: activation of MAPKK activity; JNK cascade; positive regulation of cell proliferation; Ras protein signal transduction

NCBI Summary:This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
UniProt Code:P46109
NCBI GenInfo Identifier:1169094
NCBI Gene ID:1399
NCBI Accession:P46109.1
UniProt Secondary Accession:P46109,A8KA44, D3DX35,
UniProt Related Accession:P46109
Molecular Weight:33,777 Da
NCBI Full Name:Crk-like protein
NCBI Synonym Full Names:CRK like proto-oncogene, adaptor protein
NCBI Official Symbol:CRKL  
NCBI Protein Information:crk-like protein
UniProt Protein Name:Crk-like protein
Protein Family:Crk-like protein
UniProt Gene Name:CRKL  
UniProt Entry Name:CRKL_HUMAN
Anti-CRKL Antibody (CAB0511)Western blot analysis of extracts of various cell lines, using CRKL antibody (CAB0511) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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