Anti-CD11A Antibody (CAB1644)

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SKU:
CAB1644
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-CD11A Antibody
Antibody SKU:CAB1644
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-306 of human CD11A (NP_001107852.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HepG2, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-306 of human CD11A (NP_001107852.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YNLD VRGA RSFS PPRA GRHF GYRV LQVG NGVI VGAP GEGN STGS LYQC QSGT GHCL PVTL RGSN YTSK YLGM TLAT DPTD GSIL FAAV QFST SYKT EFDF SDYV KRKD PDAL LKHV KHML LLTN TFGA INYV ATEV FREE LGAR PDAT KVLI IITD GEAT DSGN IDAA KDII RYII GIGK HFQT KESQ ETLH KFAS KPAS EFVK ILDT FEKL KDLF TELQ KKIY VIEG TSKQ DLTS FNME LSSS GISA DLSR GHAV VGAV GAKD WAGG FLDL KADL QDDT F
Gene ID:3683
Uniprot:P20701
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:119kDa/128kDa/134kDa
Observed MW:175kDa
Synonyms:CD11A, LFA-1, LFA1A, CD11a, ITGAL
Background:ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; immunological synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function:protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; activated T cell proliferation; positive regulation of calcium-mediated signaling; signal transduction; receptor clustering; heterophilic cell adhesion; leukocyte adhesion; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of T cell proliferation; inflammatory response; blood coagulation; cell adhesion; cell motility; leukocyte migration

NCBI Summary:ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P20701
NCBI GenInfo Identifier:88911345
NCBI Gene ID:3683
NCBI Accession:P20701.3
UniProt Secondary Accession:P20701,O43746, Q45H73, Q96HB1, Q9UBC8,
UniProt Related Accession:P20701
Molecular Weight:
NCBI Full Name:Integrin alpha-L
NCBI Synonym Full Names:integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
NCBI Official Symbol:ITGAL  
NCBI Official Synonym Symbols:CD11A; LFA-1; LFA1A  
NCBI Protein Information:integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide
UniProt Protein Name:Integrin alpha-L
UniProt Synonym Protein Names:CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain; CD_antigen: CD11a
Protein Family:Integrin
UniProt Gene Name:ITGAL  
UniProt Entry Name:ITAL_HUMAN
Anti-CD11A Antibody (CAB1644)Western blot analysis of extracts of various cell lines, using CD11A antibody (CAB1644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CD11A Antibody (CAB1644)Immunohistochemistry of paraffin-embedded rat spleen using CD11A antibody (CAB1644) at dilution of 1:100 (40x lens).
Anti-CD11A Antibody (CAB1644)Immunohistochemistry of paraffin-embedded human appendix using CD11A antibody (CAB1644) at dilution of 1:100 (40x lens).
Anti-CD11A Antibody (CAB1644)Immunohistochemistry of paraffin-embedded mouse spleen using CD11A antibody (CAB1644) at dilution of 1:100 (40x lens).
Anti-CD11A Antibody (CAB1644)Immunofluorescence analysis of rat spleen using CD11A Polyclonal Antibody (CAB1644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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