Anti-BFAR Antibody (CAB8308)
- SKU:
- CAB8308
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | BFAR Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8308 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human BFAR (NP_057645.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, SKOV3, A-549, SH-SY5Y, SW480, Mouse brain, Mouse heart, Mouse liver, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human BFAR (NP_057645.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEEP QKSY VNTM DLER DEPL KSTG PQIS VSEF SCHC CYDI LVNP TTLN CGHS FCRH CLAL WWAS SKKT ECPE CREK WEGF PKVS ILLR DAIE KLFP DAIR LRFE DIQQ NNDI VQSL AAFQ KYGN DQIP LAPN TGRA NQQM |
| Gene ID: | 51283 |
| Uniprot: | Q9NZS9 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 38kDa/52kDa |
| Observed MW: | 53kDa |
| Synonyms: | BFAR, BAR, RNF47 |
| Background: |
| UniProt Protein Function: | BFAR: Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Apoptosis; Ubiquitin conjugating system; Membrane protein, integral Chromosomal Location of Human Ortholog: 16p13.12 Cellular Component: endoplasmic reticulum; membrane Molecular Function:protein binding; protein binding, bridging Biological Process: negative regulation of apoptosis; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process |
| UniProt Code: | Q9NZS9 |
| NCBI GenInfo Identifier: | 74753089 |
| NCBI Gene ID: | 51283 |
| NCBI Accession: | Q9NZS9.1 |
| UniProt Secondary Accession: | Q9NZS9,A8K4Z9, B4DUT0, D3DUG8, |
| UniProt Related Accession: | Q9NZS9 |
| Molecular Weight: | 38,652 Da |
| NCBI Full Name: | Bifunctional apoptosis regulator |
| NCBI Synonym Full Names: | bifunctional apoptosis regulator |
| NCBI Official Symbol: | BFAR |
| NCBI Official Synonym Symbols: | BAR; RNF47 |
| NCBI Protein Information: | bifunctional apoptosis regulator |
| UniProt Protein Name: | Bifunctional apoptosis regulator |
| UniProt Synonym Protein Names: | RING finger protein 47 |
| Protein Family: | Bifunctional apoptosis regulator |
| UniProt Gene Name: | BFAR |
| UniProt Entry Name: | BFAR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BFAR antibody (CAB8308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using Anti-BFAR Polyclonal Antibody (CAB8308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using Anti-BFAR Polyclonal Antibody (CAB8308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Anti-BFAR Polyclonal Antibody (CAB8308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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