Anti-BCAT2 Antibody (CAB7426)

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SKU:
CAB7426
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-BCAT2 Antibody
Antibody SKU:CAB7426
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human BCAT2 (NP_001181.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, Jurkat, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human BCAT2 (NP_001181.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LLCG PRRY ASSS FKAA DLQL EMTQ KPHK KPGP GEPL VFGK TFTD HMLM VEWN DKGW GQPR IQPF QNLT LHPA SSSL HYSL QLFE GMKA FKGK DQQV RLFR PWLN MDRM LRSA MRLC LPSF DKLE LLEC IRRL IEVD KDWV PDAA GTSL YVRP VLIG NEPS LGVS QPTR ALLF VILC PVGA Y
Gene ID:587
Uniprot:O15382
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:33kDa/44kDa
Observed MW:39KDa
Synonyms:BCAT2, BCAM, BCATM, BCT2, PP18
Background:This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; EC 2.6.1.42; Mitochondrial; Transferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:branched-chain-amino-acid transaminase activity

Biological Process: valine metabolic process; branched chain family amino acid catabolic process; leucine metabolic process; branched chain family amino acid biosynthetic process; isoleucine catabolic process

NCBI Summary:This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Code:O15382
NCBI GenInfo Identifier:20455470
NCBI Gene ID:587
NCBI Accession:O15382.2
UniProt Secondary Accession:O15382,O00269, Q96KG1, Q9BTB6, Q9BUU6, B2RB87,
UniProt Related Accession:O15382
Molecular Weight:33,777 Da
NCBI Full Name:Branched-chain-amino-acid aminotransferase, mitochondrial
NCBI Synonym Full Names:branched chain amino-acid transaminase 2, mitochondrial
NCBI Official Symbol:BCAT2  
NCBI Official Synonym Symbols:BCAM; BCT2; PP18; BCATM  
NCBI Protein Information:branched-chain-amino-acid aminotransferase, mitochondrial; placental protein 18; branched chain aminotransferase 2, mitochondrial
UniProt Protein Name:Branched-chain-amino-acid aminotransferase, mitochondrial
UniProt Synonym Protein Names:Placental protein 18; PP18
UniProt Gene Name:BCAT2  
UniProt Entry Name:BCAT2_HUMAN
Anti-BCAT2 Antibody (CAB7426)Western blot analysis of extracts of various cell lines, using BCAT2 antibody (CAB7426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-BCAT2 Antibody (CAB7426)Western blot analysis of extracts of various cell lines, using BCAT2 antibody (CAB7426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-BCAT2 Antibody (CAB7426)Immunohistochemistry of paraffin-embedded rat brain using BCAT2 Rabbit pAb (CAB7426) at dilution of 1:100 (40x lens).
Anti-BCAT2 Antibody (CAB7426)Immunohistochemistry of paraffin-embedded mouse kidney using BCAT2 Rabbit pAb (CAB7426) at dilution of 1:100 (40x lens).
Anti-BCAT2 Antibody (CAB7426)Immunofluorescence analysis of NIH-3T3 cells using BCAT2 Rabbit pAb (CAB7426) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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