Anti-AVPR2 Antibody (CAB7879)
- SKU:
- CAB7879
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AVPR2 Antibody |
| Antibody SKU: | CAB7879 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 232-371 of human AVPR2 (NP_000045.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293T, COS-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 232-371 of human AVPR2 (NP_000045.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IHAS LVPG PSER PGGR RRGR RTGS PGEG AHVS AAVA KTVR MTLV IVVV YVLC WAPF FLVQ LWAA WDPE APLE GAPF VLLM LLAS LNSC TNPW IYAS FSSS VSSE LRSL LCCA RGRT PPSL GPQD ESCT TASS SLAK DTSS |
| Gene ID: | 554 |
| Uniprot: | P30518 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 33kDa/40kDa |
| Observed MW: | 46kDa |
| Synonyms: | AVPR2, ADHR, DI1, DIR, DIR3, NDI, V2R |
| Background: | This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. |
| UniProt Protein Function: | AVPR2: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption. Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD). This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI); also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Receptor, GPCR; Transporter; Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq28 Cellular Component: endoplasmic reticulum; endosome; Golgi apparatus; integral to membrane; integral to plasma membrane; plasma membrane Molecular Function:peptide binding; protein binding; vasopressin receptor activity Biological Process: adenylate cyclase activation; cellular response to hormone stimulus; excretion; G-protein signaling, coupled to cAMP nucleotide second messenger; hemostasis; I-kappaB kinase/NF-kappaB cascade; interferon-gamma production; positive regulation of cell proliferation; positive regulation of protein ubiquitination; positive regulation of systemic arterial blood pressure; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure by vasopressin; renal water homeostasis; response to cytokine stimulus; telencephalon development; transmembrane transport; water transport Disease: Diabetes Insipidus, Nephrogenic, X-linked; Nephrogenic Syndrome Of Inappropriate Antidiuresis |
| NCBI Summary: | This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30518 |
| NCBI GenInfo Identifier: | 267256 |
| NCBI Gene ID: | 554 |
| NCBI Accession: | P30518.1 |
| UniProt Secondary Accession: | P30518,O43192, Q3MJD3, Q9UCV9, C5HF20, |
| UniProt Related Accession: | P30518 |
| Molecular Weight: | |
| NCBI Full Name: | Vasopressin V2 receptor |
| NCBI Synonym Full Names: | arginine vasopressin receptor 2 |
| NCBI Official Symbol: | AVPR2 |
| NCBI Official Synonym Symbols: | DI1; DIR; NDI; V2R; ADHR; DIR3 |
| NCBI Protein Information: | vasopressin V2 receptor |
| UniProt Protein Name: | Vasopressin V2 receptor |
| UniProt Synonym Protein Names: | AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor |
| Protein Family: | Vasopressin V2 receptor |
| UniProt Gene Name: | AVPR2 |
| UniProt Entry Name: | V2R_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AVPR2 Antibody (CAB7879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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