Anti-ASPSCR1 항체 (CAB7481)

Antibody Name:	Anti-ASPSCR1 Antibody
Antibody SKU:	CAB7481
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SW480, A-549, BT-474, Mouse liver, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KSKS GQDP QQEQ EQER ERDP QQEQ ERER PVDR EPVD REPV VCHP DLEE RLQA WPAE LPDE FFEL TVDD VRRR LAQL KSER KRLE EAPL VTKA FREA QIKE KLER YPKV ALRV LFPD RYVL QGFF RPSE TVGD LRDF VRSH LGNP ELSF YLFI TPPK TVLD DHTQ TLFQ ANLF PAAL VHLG AEEP AGVY LEPG LLEH AISP SAAD VLVA RYMS RAAG SPSP LPAP DPAP KSEP AAEE GALV PPEP IPGT AQPV KRSL GKVP KWLK LPAS KR
Gene ID:	79058
Uniprot:	Q9BZE9
Cellular Location:	Cytoplasm, Endomembrane system, Endoplasmic reticulum-Golgi intermediate compartment membrane, Nucleus, Peripheral membrane protein
Calculated MW:	37kDa/54kDa/60kDa/69kDa
Observed MW:	80kDa

Synonyms:

ASPSCR1, ASPCR1, ASPL, ASPS, RCC17, TUG, UBXD9, UBXN9

Background:

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.

UniProt Protein Function:	ASPSCR1: Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface. Interacts with GLUT4. Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; Membrane protein, peripheral; Vesicle Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; plasma membrane Molecular Function:protein binding Disease: Alveolar Soft Part Sarcoma
NCBI Summary:	The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011]
UniProt Code:	Q9BZE9
NCBI GenInfo Identifier:	74717746
NCBI Gene ID:	79058
NCBI Accession:	Q9BZE9.1
UniProt Secondary Accession:	Q9BZE9,Q7Z6N7, Q8WV59, Q96LS5, Q96M40, A8K3K9,
UniProt Related Accession:	Q9BZE9
Molecular Weight:	37,910 Da
NCBI Full Name:	Tether containing UBX domain for GLUT4
NCBI Synonym Full Names:	ASPSCR1, UBX domain containing tether for SLC2A4
NCBI Official Symbol:	ASPSCR1
NCBI Official Synonym Symbols:	TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
NCBI Protein Information:	tether containing UBX domain for GLUT4
UniProt Protein Name:	Tether containing UBX domain for GLUT4
UniProt Synonym Protein Names:	Alveolar soft part sarcoma chromosomal region candidate gene 1 protein; Alveolar soft part sarcoma locus; Renal papillary cell carcinoma protein 17; UBX domain-containing protein 9
Protein Family:	Tether containing UBX domain
UniProt Gene Name:	ASPSCR1
UniProt Entry Name:	ASPC1_HUMAN

	Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (CAB7481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (CAB7481) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (CAB7481) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (CAB7481) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (CAB7481) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)