Anti-AKAP4 Antibody (CAB14813)
- SKU:
- CAB14813
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AKAP4 Antibody |
| Antibody SKU: | CAB14813 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QSPS APPA KPPS TQRA VISP DGEC SIDD LSFY VNRL SSLV IQMA HKEI KEKL EGKS KCLH HSIC PSPG NKER ISPR TPAS KIAS EMAY EAVE LTAA EMRG TGEE SREG GQKS FLYS ELSN KSKS GDKQ MSQR ESKE FADS ISKG LMVY ANQV |
| Gene ID: | 8852 |
| Uniprot: | Q5JQC9 |
| Cellular Location: | Cell projection, cilium, flagellum |
| Calculated MW: | 93kDa/94kDa |
| Observed MW: | 82kDa |
| Synonyms: | AKAP4, AKAP 82, AKAP-4, AKAP82, CT99, FSC1, HI, PRKA4, hAKAP82, p82 |
| Background: | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | AKAP4: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Belongs to the AKAP110 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA); Adaptor/scaffold Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: cytoskeleton; perinuclear region of cytoplasm; cytoplasm; nucleus; Z disc; cAMP-dependent protein kinase complex Molecular Function:protein kinase A binding Biological Process: protein localization; sperm motility; cell projection organization and biogenesis; single fertilization; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility; signal transduction |
| NCBI Summary: | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q5JQC9 |
| NCBI GenInfo Identifier: | 21493037 |
| NCBI Gene ID: | 8852 |
| NCBI Accession: | NP_003877.2 |
| UniProt Secondary Accession: | Q5JQC9,O60904, O95246, Q5JQD1, Q5JQD2, Q5JQD3, A0AV85 |
| UniProt Related Accession: | Q5JQC9 |
| Molecular Weight: | 94,477 Da |
| NCBI Full Name: | A-kinase anchor protein 4 isoform 1 |
| NCBI Synonym Full Names: | A kinase (PRKA) anchor protein 4 |
| NCBI Official Symbol: | AKAP4 |
| NCBI Official Synonym Symbols: | HI; p82; CT99; FSC1; PRKA4; AKAP-4; AKAP82; AKAP 82; hAKAP82 |
| NCBI Protein Information: | A-kinase anchor protein 4; testis-specific gene HI; cancer/testis antigen 99; A-kinase anchor protein 82 kDa; major sperm fibrous sheath protein; protein kinase A anchoring protein 4; protein kinase A-anchoring protein 4 |
| UniProt Protein Name: | A-kinase anchor protein 4 |
| UniProt Synonym Protein Names: | A-kinase anchor protein 82 kDa; AKAP 82; hAKAP82; Major sperm fibrous sheath protein; HI; Protein kinase A-anchoring protein 4; PRKA4 |
| Protein Family: | A-kinase anchor protein |
| UniProt Gene Name: | AKAP4 |
| UniProt Entry Name: | AKAP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AKAP4 antibody (CAB14813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3s. |
 | Immunofluorescence analysis of Rat testis using AKAP4 antibody (CAB14813) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of Mouse testis using AKAP4 antibody (CAB14813) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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