Anti-ADK 항체 (CAB15023)

Antibody Name:	Anti-ADK Antibody
Antibody SKU:	CAB15023
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-345 of human ADK (NP_001114.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, A-431, Mouse testis, Mouse kidney, Mouse spleen, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-345 of human ADK (NP_001114.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PFIS QFYK ESLM KVMP YVDI LFGN ETEA ATFA REQG FETK DIKE IAKK TQAL PKMN SKRQ RIVI FTQG RDDT IMAT ESEV TAFA VLDQ DQKE IIDT NGAG DAFV GGFL SQLV SDKP LTEC IRAG HYAA SIII RRTG CTFP EKPD FH
Gene ID:	132
Uniprot:	P55263
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	34kDa/36kDa/38kDa/40kDa
Observed MW:	41kDa

Synonyms:

ADK, AK

Background:

This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.20 Chromosomal Location of Human Ortholog: 10q22\|10q11-q24 Cellular Component: cytoplasm; cytosol; nucleoplasm Molecular Function:adenosine kinase activity Biological Process: purine salvage; ribonucleoside monophosphate biosynthetic process Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency
NCBI Summary:	This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	P55263
NCBI GenInfo Identifier:	6840802
NCBI Gene ID:	132
NCBI Accession:	P55263.2
UniProt Secondary Accession:	P55263,O00741, O00742, Q16710, Q5JQ10, Q5JQ11, Q9BTN2 B7Z783, B7Z800,
UniProt Related Accession:	P55263
Molecular Weight:	36,580 Da
NCBI Full Name:	Adenosine kinase
NCBI Synonym Full Names:	adenosine kinase
NCBI Official Symbol:	ADK
NCBI Official Synonym Symbols:	AK
NCBI Protein Information:	adenosine kinase
UniProt Protein Name:	Adenosine kinase
UniProt Synonym Protein Names:	Adenosine 5'-phosphotransferase
UniProt Gene Name:	ADK
UniProt Entry Name:	ADK_HUMAN

	Western blot analysis of extracts of various cell lines, using ADK antibody (CAB15023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of NIH/3T3 cells using ADK Rabbit pAb (CAB15023) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using ADK Rabbit pAb (CAB15023) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using ADK Rabbit pAb (CAB15023) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-ADK Antibody (CAB15024)

Anti-ADK Antibody (CAB19992)[KO Validated]

ADK Antibody (PACO06002)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)