Anti-ACADVL Antibody (CAB7865)
- SKU:
- CAB7865
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ACADVL Antibody |
| Antibody SKU: | CAB7865 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ACADVL (NP_000009.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, LO2, A-549, U-87MG, HeLa, SW620, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ACADVL (NP_000009.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQAA RMAA SLGR QLLR LGGG SSRL TALL GQPR PGPA RRPY AGGA AQLA LDKS DSHP SDAL TRKK PAKA ESKS FAVG MFKG QLTT DQVF PYPS VLNE EQTQ FLKE LVEP VSRF FEEV NDPA KNDA LEMV EETT WQGL KELG |
| Gene ID: | 37 |
| Uniprot: | P49748 |
| Cellular Location: | Mitochondrion inner membrane |
| Calculated MW: | 68kDa/70kDa/72kDa |
| Observed MW: | 70kDa |
| Synonyms: | ACADVL, ACAD6, LCACD, VLCAD |
| Background: | The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | BAZ1A: Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end- positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression. Component of the ACF chromatin remodeling complex that includes BAZ1A and SMARCA5. Additional this complex can form, together with CHRAC1 and POLE1, the histone-fold protein complex, CHRAC. Interacts with NCOR1 (via its RD1 domain); the interaction corepresses a number of NCOR1-regulated genes. Highly expressed in testis and at low or undetectable levels in other tissues analyzed. Belongs to the WAL family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, protein; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); DNA replication; DNA-binding; ATYPICAL group; BAZ family Chromosomal Location of Human Ortholog: 14q13.2 Cellular Component: nuclear chromosome; chromatin accessibility complex Molecular Function:protein binding; histone acetyltransferase activity; zinc ion binding Biological Process: chromatin remodeling; transcription, DNA-dependent; regulation of transcription, DNA-dependent; DNA-dependent DNA replication |
| NCBI Summary: | The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49748 |
| NCBI GenInfo Identifier: | 4557235 |
| NCBI Gene ID: | 37 |
| NCBI Accession: | NP_000009 |
| UniProt Secondary Accession: | P49748,O76056, Q8WUL0, B4DEB6, F5H2A9, |
| UniProt Related Accession: | P49748 |
| Molecular Weight: | 68.5 kDa (636aa) |
| NCBI Full Name: | very long-chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1 |
| NCBI Synonym Full Names: | acyl-CoA dehydrogenase very long chain |
| NCBI Official Symbol: | ACADVL |
| NCBI Official Synonym Symbols: | ACAD6; LCACD; VLCAD |
| NCBI Protein Information: | very long-chain specific acyl-CoA dehydrogenase, mitochondrial |
| UniProt Protein Name: | Bromodomain adjacent to zinc finger domain protein 1A |
| UniProt Synonym Protein Names: | ATP-dependent chromatin-remodeling protein; ATP-utilizing chromatin assembly and remodeling factor 1; hACF1; CHRAC subunit ACF1; Williams syndrome transcription factor-related chromatin-remodeling factor 180; WCRF180; hWALp1 |
| Protein Family: | Bromodomain adjacent to zinc finger domain protein |
| UniProt Gene Name: | BAZ1A |
| UniProt Entry Name: | BAZ1A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACADVL antibody (CAB7865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using ACADVL antibody (CAB7865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |