Anti-ACAD9 Antibody (CAB7798)
- SKU:
- CAB7798
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ACAD9 Antibody |
| Antibody SKU: | CAB7798 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACAD9 (NP_054768.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SKOV3, U-251MG, MCF7, 293T, NCI-H460, Mouse kidney, Mouse liver, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACAD9 (NP_054768.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGC GLFL RTTA AARA CRGL VVST ANRR LLRT SPPV RAFA KELF LGKI KKKE VFPF PEVS QDEL NEIN QFLG PVEK FFTE EVDS RKID QEGK IPDE TLEK LKSL GLFG LQVP EEYG GLGF SNTM YSRL GEII SMDG SITV TLAA HQAI GLKG IILA GTEE QKAK YLPK LASG EHIA AFCL TEPA SGSD AASI RSRA TLSE DKKH YILN GSKV WITN GGLA NIFT VFAK TEVV DSDG SVKD KITA FIVE RDFG GVTN GKPE DKLG IRGS NT |
| Gene ID: | 28976 |
| Uniprot: | Q9H845 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 68kDa |
| Observed MW: | 69kDa |
| Synonyms: | ACAD9, NPD002 |
| Background: | This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | ACAD9: Has a dehydrogenase activity on palmitoyl-CoA (C16:0) and stearoyl-CoA (C18:0). It is three times more active on palmitoyl-CoA then on stearoyl-CoA. Has little activity on octanoyl-CoA (C8:0), butyryl-CoA (C4:0) or isovaleryl-CoA (5:0). Belongs to the acyl-CoA dehydrogenase family. |
| UniProt Protein Details: | Protein type:EC 1.3.99.-; Mitochondrial; Oxidoreductase Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: dendrite; mitochondrial inner membrane; mitochondrion; nucleus Molecular Function:acyl-CoA binding; acyl-CoA dehydrogenase activity; electron carrier activity; FAD binding; protein binding Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase; lipid homeostasis; mitochondrial respiratory chain complex I assembly Disease: Acyl-coa Dehydrogenase Family, Member 9, Deficiency Of |
| NCBI Summary: | This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010] |
| UniProt Code: | Q9H845 |
| NCBI GenInfo Identifier: | 21361497 |
| NCBI Gene ID: | 28976 |
| NCBI Accession: | |
| UniProt Secondary Accession: | Q9H845,Q8WXX3, D3DNB8, |
| UniProt Related Accession: | Q9H845 |
| Molecular Weight: | 69kDa |
| NCBI Full Name: | acyl-CoA dehydrogenase family member 9, mitochondrial |
| NCBI Synonym Full Names: | acyl-CoA dehydrogenase family member 9 |
| NCBI Official Symbol: | ACAD9 |
| NCBI Official Synonym Symbols: | NPD002 |
| NCBI Protein Information: | acyl-CoA dehydrogenase family member 9, mitochondrial |
| UniProt Protein Name: | Acyl-CoA dehydrogenase family member 9, mitochondrial |
| Protein Family: | Acyl-CoA dehydrogenase family |
| UniProt Gene Name: | ACAD9 |
| UniProt Entry Name: | ACAD9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACAD9 antibody (CAB7798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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